肿瘤抑素抗肿瘤相关肽治疗人喉鳞状细胞癌裸鼠模型的实验研究

Anti-tumor peptide of tumstatin in the treatment of the transplantable model of human laryngeal squamous carcinoma in nude mice

目的:研究肿瘤抑素抗肿瘤相关肽对裸鼠喉癌皮下移植模型的抑瘤作用,探讨其抑瘤机制及人喉癌生物治疗新方法。方法:建立人喉癌Hep-Ⅱ细胞株裸鼠皮下接种模型,应用肿瘤抑素抗肿瘤相关肽治疗,观察肿瘤生长情况。对治疗后的肿瘤组织进行病理学检查、免疫组织化学检查及电镜观察,记数微血管密度。结果:在治疗组与对照组之间,平均鼠净重、瘤重、瘤体积及瘤重/鼠净重比,差异均有统计学意义(均P<0.01),治疗组抑瘤率为51.58%。病理学检查及电镜观察表明,治疗组肿瘤生长受到抑制,细胞分裂少见,可见肿瘤细胞的坏死凋亡,新生血管明显减少,其微血管密度明显低于对照组,差异有统计学意义(P<0.01)。结论:肿瘤抑素抗肿瘤相关肽可显著抑制人喉癌裸鼠模型肿瘤的生长和发展。

Objective：To study the effect of anti-tumor peptide of tumstatin on tumor growth of human laryngeal squamous carcinoma in nude mice and the underlying mechanism.Method：Nude mice model bearing laryngocarcinoma were established by using human laryngeal squamous carcinoma cell line（Hep-Ⅱ）.The animals were given tumstatin or PBS for 10 consecutive days.The volumes of the subcutaneous tumor were observed.The microstructure in which the general 2-step immuohistochemical examination was adopted and ultra-micro-structural changes of carcinoma after administration of tumstatin were observed under light and electron microscopes for pathology examination.Result：The differences was statistically significant in the net mice weight,tumor weight,tumor volume and tumor weight/net mice weight between the treatment group and the control group（P〈0.01）.The restrained percentage of tumor was 51.58%.The necrosis and apoptosis of the tumor cells and the angiogenesis reduction were found under light and electron microscope in the treatment group.MVD of the treatment group was lower than that of the control group（P〈0.01）.Conclusion：Tumstatin can significantly restrain the development of laryngocarcinoma.