TrkB及其配体BDNF在卵巢上皮癌中的表达及其临床意义

Expression and clinical significance of TrkB and its ligand BDNF in epithelial ovarian cancer

目的检测酪氨酸激酶B(tyrosine kinase,TrkB)及其配体脑源性神经营养因子(brainderived neurotrophic factor,BDNF)在卵巢上皮癌(EOC)中的表达,并探讨其临床意义。方法选择中山大学附属肿瘤医院病理存档的石蜡标本108例,其中10例正常卵巢、17例良性卵巢肿瘤、21例卵巢交界性肿瘤和60例卵巢上皮癌,采用免疫组化链霉素抗生物素蛋白-过氧化物酶法(SP法)检测TrkB及其配体BDNF蛋白的表达水平,分析其与临床病理因素之间的关系。结果 TrkB和BDNF蛋白在正常卵巢组织和良性卵巢上皮性肿瘤中无表达;在交界性和卵巢上皮性癌中的阳性表达率分别为19.0%、71.7%和14.0%、51.7%(P<0.05)。TrkB和BDNF蛋白在EOC组中的表达水平与其FIGO分期及病理分级有关,在Ⅲ期+Ⅳ期、低分化组(G3)比Ⅰ期+Ⅱ期、高中分化(G1、G2)组表达率高(P<0.05)。TrkB和BDNF在卵巢上皮癌中的表达水平呈正相关(r=0.428,P<0.05)。结论卵巢上皮癌中存在TrkB和BDNF的异常高表达。TrkB和BDNF可能协同作用促进了卵巢上皮癌的发生、发展,并有望成为卵巢上皮癌基因治疗的新靶点。

Objective The current study was to explore the expression profile of TrkB/BDNF in ovarian epithelial cancer and their correlation with clinical pathology variables respectively.Methods TrkB/BDNF were examined in 10 normal ovarian,17 benign ovarian epithelial tumor,21 borderline ovarian epithelial tumor and 60 ovarian epithelial cancer tissue samples by immunohistochemical Streptomycin avidin-peroxidase（SP）method.Results The expression of TrkB and BDNF were negative in normal ovarian tissue and benign epithelial tumors,and the expression rate of TrkB and BDNF in borderline ovarian tumors and ovarian epithelial cancers（EOC） was 19.0%,71.7% and 14.0%,51.7%（P0.05）.The degree of TrkB/BDNF express was more strong in G3,Ⅲ＋Ⅳ stage EOC than that in G1＋G2,Ⅰ＋Ⅱ stage EOC（P0.05）.TrkB expression was positively correlated with BDNF level in epithelial ovarian cancer（r=0.43,P0.05）.Conclusions There was a overexpression of TrkB/BDNF in ovarian epithelial cancer.TrkB may synergy with BDNF to promote tumorigenesis and development of epithelial ovarian cancer,it may be a target for EOC gene therapy.