柴达木枸杞和黑果枸杞中甜菜碱的测定

Determination of Betaine in Lycium ruthenicum Murr.and Lycium barbarum L.

采用反相高效液相色谱法测定柴达木枸杞和黑果枸杞中甜菜碱的含量。以乙腈∶水(83∶17,V/V)为流动相,Hypersil NH2(250mm×4.6mm,5μm)为色谱柱,流速0.7mL/min,检测波长195nm,柱温30℃,外标法定量。结果表明,甜菜碱线性范围为2.94—29.40μg(r=0.9987),枸杞和黑果枸杞的平均回收率(n=5)分别为98.57%和99.07%。不同产地枸杞和黑果枸杞甜菜碱含量存有差异。该方法操作简便、重现性好,适用于测定枸杞和黑果枸杞甜菜碱含量。

A new method for determination of betaine in Lycium barbarum L.and Lycium ruthenicum Murr.by RP-HPLC was established.The HPLC conditions were as follows： Hypersil NH2（250mm×4.6mm,5μm） chromatographic column,the mobile phase was acetonitrile-water（83∶17,V/V） at 30℃,the flow rate was 0.7mL/min,and the UV detection wavelength was at 195nm.The good linearity of betaine was obtained over the ranges of 2.94—29.40μg（r=0.9987）,the average recovery of Lycium barbarum L.was 98.57%（n=5） and the average recovery of Lycium ruthenicum Murr.was 99.07%（n=5）.The contents of betaine of Lycium barbarum and Lycium ruthenicum were different from different habitats.The method is simple and reproducible and it can be used to determine betaine in Lycium barbarum L.and Lycium ruthenicum Murr..