吖啶黄-纳米金荧光共振能量转移体系增敏法检测高半胱氨酸

Determination of Homocystein by Fluorescence Resonance Energy Transfer Between Acridine Yellow and Au Nanoparticles

以吖啶黄(Acridine Yellow,AY)为供体,纳米金(AuNPs)为受体建立了荧光共振能量转移(FRET)的新体系。体系中AY以非共价的方式吸附在AuNPs上,两者之间发生了有效的能量转移,导致AY的荧光猝灭。由于高半胱氨酸(Homocysteine,Hcys)和纳米金之间可以形成很强的Au-S键,使AY从AuNPs表面释放,进而使体系的荧光恢复。基于增强的荧光信号建立了检测高半胱氨酸的新方法。在最优化的条件下,检测高半胱氨酸的线性范围为5.0×10-10—1.5×10-8mol/L,检出限为0.2×10-10mol/L。

The new fluorescence resonance energy transfer（FRET） system was established with acridine yellow（AY） as donors and Au nanoparticles（AuNPs） as acceptors.AY adsorbed on AuNPs at noncovalent patten in the system,and there was effectively energy transfer between them,that resulted in fluorescence quenching of AY.Because of forming strong Au—S bond between homocysteine and AuNPs,AY was released from surface of AuNPs,then the fluorescence of system recovered.The new method for the determination of homocysteine was established based on enhanced fluorescence signal.The linear range of detected homocysteine was from 5.0×10-10—1.5×10-8mol/L with detection limit of 0.2×10-10mol/L under the optimal conditions.