万寿菊组织培养体系的建立

Study on the Tissue Culture System of Tagetes erecta L.

以万寿菊为试材,采用叶片和茎段作为外植体,研究其组织培养不同阶段的最佳培养基,以建立万寿菊组织培养技术体系。结果表明:叶片是诱导愈伤组织的良好材料,适合叶片诱导愈伤组织的培养基是MS+6-BA 2.0mg/L+NAA 0.2mg/L;适合愈伤组织分化不定芽的培养基是MS+6-BA 1.0mg/L+NAA 0.1mg/L;适合生根的培养基是1/2MS+IAA 0.8mg/L+IBA 1.0mg/L。

Using leaves and stems as explants of Tagetes erecta L. ,the optimal medium at different stages of the tissue culture was studied to establish the tissue culture system of Tagetes erecta L.. The results showed that the leaves were fit for inducing callus. In MS medium with 6-BA 2.0 mg/L and NAA 0. 2 mg/L, the callus induction rate was the highest. The optimum medium of the adventitious bud regeneration was MS medium with 6-BA 1.0 mg/L and NAA 0. 1 mg/L. In 1/2MS medium with IBA 1.0 mg/L and IAA 0. 8 mg/L,the root could be induced easily.