摘要
选用24只3月龄、体质量(13±1.0)kg的雄性波尔山羊,随机分为4组,每组6只,试验期60d。Ⅰ组为对照组(饲料添加微量元素);Ⅱ组(低剂量组)、Ⅲ组(中剂量组)、Ⅳ组(高剂量组)分别肌肉注射"生命元"0.5,1.0,2.0mL/kg。试验前1d及试验后15,30,45,60d静脉采血测定外周血液T淋巴细胞酸性α-醋酸萘酯酶阳性率(ANAE)、红细胞补体受体花环率(C3bRR)及免疫复合花环率(ICR);试验后30,45,60d测定外周血液淋巴细胞周期指标。结果表明:(1)"生命元"能提高波尔山羊血液中T淋巴细胞酸性α-醋酸萘酯酶阳性率,且在试验后45,60d试验组显著高于对照组(P<0.05或P<0.01),并以中剂量组效果最佳。(2)"生命元"能够提高波尔山羊红细胞补体受体花环率,降低红细胞的免疫复合物花环率,增强波尔山羊的红细胞免疫功能。(3)与对照组相比,试验组不同程度降低G0/G1期和DNA的百分含量,提高S、G2-M期DNA的百分含量以及细胞分裂指数(PI)和DNA百分含量,并在一定时期呈现显著性(P<0.05或P<0.01)差异,并以中剂量组效果最佳。
The experiment selected 24 health male Boer goat of 3-month-old,(13 ±1.0) kg body weight,randomly divided into 4 groups of 6 each,and the experiment lasted 60 d.GroupⅠ(the control group) added the microelement;groupⅡ(low-dose-group),groupⅢ(middle-dose-group),groupⅣ(high-dose-group) were intramuscularly injectied,with "Sheng ming yuan" at 0.5,1.0,2.0 mL/kg,respectively.At the day befor and after 15,30,45,60 d of the injecting "Sheng ming yuan",determinated the positive rate of peripheral blood T lymphocytes(ANAE),the rate of erythrocyte complement receptor rosette(E-C3bRR) and immune complex rosette(E-ICR);30,45,60 d after injecting the cycle of lymphocytes was determinated in blood.The results showed:①"Sheng ming yuan" was able to improve the rate of Boer goat's T cell ANAE,strengthen the cellular immune response,especially in the middle-dose group.②The injection improved the rate of Boer goat's E-C3bRR and E-ICR.③The injection could shorten the period of G0/G1 of lymphocyte in perpheral blood,decrease the DNA contents pecentage and increase the DNA contents percentage of the S,G2-M scheduled time and cell proliferating index(PI),compared with the control group(P0.05 or P0.01),in markedly the middle-dose-group showed the best.④"Sheng ming yuan" is able to increase Boer goat's erythrocyte complement acceptor garland rate,reduces erythrocyte's immunity compound garland rate,strengthen the immune reponse.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第3期447-450,456,共5页
Chinese Journal of Veterinary Science
基金
四川省教育厅自然科学重点资助项目(2006A009)
四川省科技厅应用基础研究资助项目(2008JY0098)
"长江学者和创新团队发展计划"创新团队资助项目(IRT0848)
关键词
生命元
波尔山羊
免疫功能
Sheng ming yuan
Boer goat
immune function