复方微量元素“生命元”对波尔山羊免疫功能的影响

Change of immune function in Boer goat by injected “Sheng ming yuan”

选用24只3月龄、体质量(13±1.0)kg的雄性波尔山羊,随机分为4组,每组6只,试验期60d。Ⅰ组为对照组(饲料添加微量元素);Ⅱ组(低剂量组)、Ⅲ组(中剂量组)、Ⅳ组(高剂量组)分别肌肉注射"生命元"0.5,1.0,2.0mL/kg。试验前1d及试验后15,30,45,60d静脉采血测定外周血液T淋巴细胞酸性α-醋酸萘酯酶阳性率(ANAE)、红细胞补体受体花环率(C3bRR)及免疫复合花环率(ICR);试验后30,45,60d测定外周血液淋巴细胞周期指标。结果表明:(1)"生命元"能提高波尔山羊血液中T淋巴细胞酸性α-醋酸萘酯酶阳性率,且在试验后45,60d试验组显著高于对照组(P<0.05或P<0.01),并以中剂量组效果最佳。(2)"生命元"能够提高波尔山羊红细胞补体受体花环率,降低红细胞的免疫复合物花环率,增强波尔山羊的红细胞免疫功能。(3)与对照组相比,试验组不同程度降低G0/G1期和DNA的百分含量,提高S、G2-M期DNA的百分含量以及细胞分裂指数(PI)和DNA百分含量,并在一定时期呈现显著性(P<0.05或P<0.01)差异,并以中剂量组效果最佳。

The experiment selected 24 health male Boer goat of 3-month-old,（13 ±1.0） kg body weight,randomly divided into 4 groups of 6 each,and the experiment lasted 60 d.GroupⅠ（the control group） added the microelement;groupⅡ（low-dose-group）,groupⅢ（middle-dose-group）,groupⅣ（high-dose-group） were intramuscularly injectied,with ＂Sheng ming yuan＂ at 0.5,1.0,2.0 mL/kg,respectively.At the day befor and after 15,30,45,60 d of the injecting ＂Sheng ming yuan＂,determinated the positive rate of peripheral blood T lymphocytes（ANAE）,the rate of erythrocyte complement receptor rosette（E-C3bRR） and immune complex rosette（E-ICR）;30,45,60 d after injecting the cycle of lymphocytes was determinated in blood.The results showed：①＂Sheng ming yuan＂ was able to improve the rate of Boer goat＇s T cell ANAE,strengthen the cellular immune response,especially in the middle-dose group.②The injection improved the rate of Boer goat＇s E-C3bRR and E-ICR.③The injection could shorten the period of G0/G1 of lymphocyte in perpheral blood,decrease the DNA contents pecentage and increase the DNA contents percentage of the S,G2-M scheduled time and cell proliferating index（PI）,compared with the control group（P0.05 or P0.01）,in markedly the middle-dose-group showed the best.④＂Sheng ming yuan＂ is able to increase Boer goat＇s erythrocyte complement acceptor garland rate,reduces erythrocyte＇s immunity compound garland rate,strengthen the immune reponse.