甘草甜素治疗重症急性胰腺炎的实验研究

Therapeutic effect of glycyrrhizin for severe acute pancreatitis in rats

目的:探讨甘草甜素对大鼠重症急性胰腺炎(SAP)疗效。方法:将大鼠随机均分为假手术组,SAP组,SAP+甘草甜素治疗组(治疗组),以5%牛磺胆酸钠胰胆管逆行注射制作大鼠SAP模型。比较各组术后的生存时间,术后不同时间点血淀粉酶及血清TNF-α,IL-1β,IL-6的水平及胰腺组织病理学评分。结果:假手术组大鼠在观察时间内无死亡,SAP组和治疗组大鼠术后的平均生存时间分别为(28.50±17.82)h和(41.50±17.59)h,两者差异有统计学意义(P<0.05);与假手术组比较,SAP组和治疗组各时间点血清TNF-α,IL-1β,IL-6水平均明显升高(均P<0.05),但治疗组上述指标升高程度均明显低于SAP组(均P<0.05);除假手术组外,SAP组和治疗组胰腺组织均呈现出不同程度的SAP病理改变,但治疗组病理改变明显较轻,各时间点组织学评分也明显低于SAP组(均P<0.05)。结论:甘草甜素治疗大鼠重症急性胰腺炎有一定的疗效。

Objective： To investigete the therapeutic effect of glycyrrhizin on severe acute pancreatitis（SAP） in rats. Methods： Rats were equally randomized into sham operation group,SAP group and SAP plus glycyrrhizin treatment group（treatment group）.The SAP model was induced by retrograde cholangiopancreatic duct injection of 5% sodium taurocholate.The postoperative survival time and the serum levels of amylase,IL-1β,IL-6 and TNF-α,as well as the pathological scores of pancreatic tissue of each group at the predefined time points after surgery were determined and compared. Results： No death occurred in the sham operation group during the observation period,while the average survival time of the SAP group and treatment group was（28.50±17.82） and（41.50±17.59） hours respectively,and the difference between them had statistical significance（P0.05）.Compared with the sham operation group,the serum levels of amylase,TNF-α,IL-1β and IL-6 of the SAP group and treatment group at each observation point were all significantly increased（all P0.05）,but the elevated levels of the above parameters in the treatment group were all significantly lower than those in the SAP group（all P0.05）.Except for the sham operation group,both the SAP group and treatment group presented the pathological features of SAP,but to a different extent;the pathological change in the treatment group was relatively mild and its pathological scores at each time points were also significantly lower than those of the SAP group（all P0.05）. Conclusion： Glycyrrhizin has a definite therapeutic effect on SAP in rats.