MicroRNA在人胰腺癌中表达谱的初步研究

Preliminary study of microRNA expression profiles in human pancreatic cancer

目的:探讨微小RNA(miRNA)在人胰腺癌中的表达谱,为进一步研究miRNA在胰腺癌发生发展中的分子机制奠定基础。方法:利用miRNA芯片技术比较胰腺癌组织和癌旁正常胰腺组织的miRNA表达差异;挑选其中两个差异表达的miRNA运用荧光定量实时聚合酶链反应(qRT-PCR)验证。结果:与正常胰腺组织相比,胰腺癌组织中表达上调超过1.5倍的miRNA有15个,表达下调超过1.5倍的miRNA有11个;qRT-PCR检测8例胰腺癌组织和相应癌旁正常胰腺组织中miR-10b的相对表达量分别为0.0743±0.0222和0.0287±0.0129;miR-21相对表达量分别为0.3062±0.1117和0.0240±0.0137(均P<0.05),与miRNA芯片结果相符合。结论:miRNA芯片筛选出的26种差异表达的miRNA可能与胰腺癌的发生发展相关,其各自在胰腺癌中的作用值得进一步研究。

Objective： To investigate the microRNAs（miRNAs） expression profiles in human pancreatic cancer,so as to provide a basis for further exploring the role of miRNAs in the molecular mechanism that leads to the occurrence and development of pancreatic cancer. Methods： The differentially expressed miRNAs between the pancreatic cancer tissue and adjacent normal pancreatic tissue were analyzed by microRNA array,and then two of the differentially expressed miRNA were selected to perform quantitative real time PCR（qRT-PCR） assay,so as to validate the microRNA array results. Results： Compared with the normal pancreatic tissues,15 miRNAs were more than 1.5-fold up-regulated,and 11 miRNAs were more than 1.5-fold down-regulated in pancreatic cancer tissues.The values of relative quantification（RQ） of miR-10b and miR-21 in pancreatic cancer tissues and their adjacent normal tissues from 8 cases detected by qRT-PCR were 0.0743±0.0222 vs.0.0287±0.0129,and 0.3062±0.1117 vs.0.0240±0.0137,respectively（both P0.05）,and these were consistent with the microRNA array results. Conclusion： The 26 differentially expressed miRNAs screened by microRNA array may contribute to the occurrence and development of pancreatic cancer,and their respective roles in this disease are worthy of further investigation.