青心酮防治ApoE(-/-)小鼠主动脉粥样硬化斑块形成与脂氧素的关系

Relationship between 3,4-Dihydroxyaceto-phenone Prevention of Atherosclerosis and Lipoxin in ApoE(-/-) mice

目的观察青心酮防治ApoE(-/-)小鼠主动脉粥样硬化(AS)病变形成与脂氧素的关系。方法取8只8周龄C57BL/6小鼠作空白对照组;取24只8周龄的ApoE(-/-)小鼠,♂,随机分成3组(每组8只):动脉硬化组(乙醇20 mg kg 1 d 1);青心酮组(青心酮20 mg kg 1 d 1);辛伐他汀组(辛伐他汀20 mg kg 1 d 1)。所有小鼠均以"西方类型膳食"饲养至16周。取血检测脂氧素、血脂和15-脂氧合酶蛋白的含量等;取主动脉根部行HE染色观察ApoE(-/-)主动脉粥样硬化病变情况;电镜观察斑块内皮细胞变化。Western blotting法测定血清中15-LO水平。结果青心酮组脂氧素增加,血脂含量降低,AS病灶形成减少,斑块内皮细胞损伤减轻,15-脂氧合酶含量减少。结论青心酮防治ApoE(-/-)小鼠AS病变,可能与其增加15-脂氧合酶途径产物——脂氧素有关。

OBJECTIVE To observe the relationship between 3,4-Dihydroxyaceto-phenone（DHAP） prevention of atherosclerosis（AS） and lipoxins（LXs） in ApoE（-/-） mice. METHODS There were 8 eight-week-old C57BL/6 mice in the normal control group; 24 eight-week-old male ApoE （-/-） mice were randomly divided into three groups： AS group （n=8, ip. alcohol 20 mg.kg-1.d-1）; DHAP treatment group （n=8, ip. DHAP 20 mg.kg-1.d-1）; simvastatin treatment group （n=8, ip. simvastatin 20 mg.kg-1.d-1）. All mice were fed with a Western diet （21% fat, 0.15% cholesterol） for 16 weeks. Their blood was collected for determination of blood lipids and lipoxin concentration. Sections of aortic root were stained by HE. The structures of cells were observed by electron microscope. Western-blotting was applied to detect 15-LO protein in serum. RESULTS The concentration of LXs increased in DHAP-treated group. The concentration of TG and TC decreased in DHAP-treated group; the formation of AS plaque was reduced; the injuries of cells decreased; 15-1ipoxygenase also increased at atherosclerosis plaque in DHAP-treated group. CONCLUSION It is suggested that DHAP could be used effectively for the prevention and treatment of AS, possibly through promoting the 15-1ipoxygenase product synthesis of anti-inflammatory lipoxin pathway.