磺丁基醚-β-环糊精的高效毛细管电泳-间接UV法分析

Analysis of Sulfobutyl Ether-β-cyclodextrin by High Performance Capillary Electrophoresis with Indirect UV Detection

建立了高效毛细管电泳-间接UV法测定药用辅料磺丁基醚-β-环糊精(SBE-β-CD)。采用未涂层熔融石英毛细管柱,以30 mmol/L苯甲酸-三羟甲基氨基甲烷(Tris)(pH 7.5)为运行缓冲液,分离电压30 kV,检测波长214 nm。考察了缓冲液类型与pH、检测波长、电压、温度和样品浓度对测定的影响,并评价了不同批次的SBE-β-CD。

An high performance capillary electrophoresis method with indirect UV detection was established for the analysis of sulfobutyl ether-β-cyclodextrin. An uncoated fused-silica capillary column was used with the running buffer of 30 mmol/L benzoic acid-Tris （pH 7.5） at the applied voltage of 30 kV and detection wavelength of 214 nm. The effects of running buffer, pH, detection wavelength, applied voltage, temperature and sample concentration on the analysis were investigated. Samples from different batches were evaluated.