丁胱亚磺酰亚胺联合放射线对食管癌细胞株TE-1的增敏效应

Radiosensitizative Effect on Esophageal Cell Line TE-1 by BSO Combined Radiation

目的研究丁胱亚磺酰亚胺(buthionine sulfoximine,BSO)联合放射线对食管癌细胞株TE-1放射增敏的影响。方法四甲基偶氮唑蓝比色实验(MTT法)观察BSO及放射线对细胞增殖的抑制作用及BSO对食管癌细胞株TE-1的放射增敏作用。流式细胞法观察BSO、放射线对细胞凋亡及细胞周期的影响。反转录聚合酶链反应(reverse transcription polymerase chine reaction,RT-PCR)、Western blot法观察BSO对锰超氧化物歧化酶(manganese superoxide dismutase,MnSOD)mRNA及蛋白表达的影响。结果 BSO能抑制食管癌TE-1细胞的增殖,具有明显的量效和时效关系;BSO对食管癌TE-1细胞有明显的放射增敏作用;BSO与放射线联合作用于TE-1细胞后,G2/M期细胞比例及凋亡率明显增加,MnSOD mRNA及蛋白表达下降。结论 BSO可能通过影响TE-1细胞周期,降低MnSOD mR-NA及蛋白的表达,从而抑制食管癌细胞增殖、诱导凋亡来增强放射线对食管癌细胞的杀伤作用。

Objective To explore radiosensitizative effect on esophageal cell line TE-1 by buthionine sulfoximine（BSO）combined with radiation.Methods Colorimetric 3-[4,5-dimethy thiazol-2-yl]-2,5-diphenyltetrazolium bromide（MTT）assay was used to study the inhibitory effect and rediosensitivity on proliferation of TE-1 cell treated with BSO and radiation.Additionally,flow cytometry,reverse transcription polymerase chine reaction（RT-PCR）and Western blot were also conducted to observe the influence of BSO and radiation on cell apoptosis,cell cycle and the expression of manganese superoxide dismutase（MnSOD）protein.Results With a clear dose and time effect,BSO could definitely inhibit the proliferation of esophageal cell and enhanced radiosensitivity of TE-1 cells.Flow cytometry analysis obviously increased cell apoptosis and G2/M phase in the effect of BSO and radiation.The expression of MnSOD mRNA or protein was decreased in the action of BSO and radiation by using RT-PCR and Western blot.Conclusion BSO could enhance rediosensitivity of esophageal cancer cell by its effect on cell cycle,MnSOD m RNA or protein.