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人胃癌细胞乙酰肝素酶对Src激酶活化的影响

Effect of Heparanase on Src Kinases Activation in Human Gastric Carcinoma Cells
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摘要 目的探讨人胃癌细胞乙酰肝素酶与Src激酶的关系。方法利用Western blot检测Src激酶抑制剂pp2的活性、pp2对人胃癌SGC-7901细胞中乙酰肝素酶表达的影响以及乙酰肝素酶表达被沉默的人胃癌SGC-7901细胞中磷酸化Src(p-Src)激酶蛋白的表达。结果 pp2对乙酰肝素酶蛋白表达无影响,在乙酰肝素酶被沉默的人胃癌SGC-7901细胞中p-Src蛋白表达降低。结论人胃癌细胞乙酰肝素酶可能调节Src激酶的磷酸化,乙酰肝素酶-Src可能是参与人胃癌侵袭、迁移和诱导血管生成的一条信号通路。 Objective To investigate the relationship of heparanase and Src in gastric carcinoma cells.Methods Western-blot was used to detect pp2 activity,the inhibitor of Src,and its effect on heparanase expression of human gastric carcinoma SGC-7901 cells,and p-Src expression in human gastric carcinoma SGC-7901 cells with silenced Heparanase.Results pp2,the inhibitor of Src,did not affect Heparanase protein expression in human gastric carcinoma SGC-7901 cells,p-Src expression decreased in human gastric carcinoma SGC-7901 cells with silenced Heparanase.Conclusion Heparanase might regulate Src phosphorylation,and Heparanase-Src might be a signal transduction pathway of invasion,migration and angiongenesis of human gastric cancer.
作者 李连兴 韩利芳 马秀梅
机构地区 内蒙古医学院附属医院病理科 内蒙古医学院基础医学院病理学教研室 内蒙古医学院基础医学院生理学教研室
出处 《肿瘤防治研究》 CAS CSCD 北大核心 2012年第3期256-259,共4页 Cancer Research on Prevention and Treatment
基金 国家自然科学基金资助项目(30660065) 教育部春晖计划资助项目(Z2007-1-01003) 博士科研启动资金资助项目(NY2006BQ006)
关键词 胃癌 乙酰肝素酶 SRC激酶 Stomach neoplasms Heparanase Src
分类号 R735.2 [医药卫生—肿瘤]
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参考文献24

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二级参考文献34

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肿瘤防治研究
2012年 第3期

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