两种建立金黄地鼠颊囊癌模型方法的对照研究

Comparison between Two Methods of Establishing Induced Buckle Pouch Carcinogenesis Model in Syrian Golden Hamster

目的二甲基苯并蒽丙酮注射法建立金黄地鼠颊囊癌动物模型,并与传统涂抹法相比较,为颊囊癌研究提供理想的动物模型。方法随机将81只金黄地鼠分为注射组、涂抹组和空白对照组。DM-BA丙酮注射组60只地鼠,将注射浓度预设为0.5%、0.4%、0.3%、0.2%、0.1%5个组,每组12只地鼠,每周两次,于注射后每周进行大体观察、监测体重并计算肿瘤生长率,至3、6、9、12周麻醉后各处死3只,进行组织学观察;涂抹组15只地鼠,以0.5%DMBA丙酮涂抹,每周三次,至6、9、12、15、18周后麻醉处死3只;空白对照组不做任何处理,于12周全部处死行组织学观察。结果注射浓度组0.4%、0.3%、0.2%、0.1%分别在13,9,12,16周时出现约为0.8 cm×0.6 cm×0.5 cm大小的肿瘤;12周时成瘤率分别为25%、87%、42%、58%;死亡率分别为48%、11%、13%、10%;相比0.5%DMBA丙酮涂抹组,12周时成瘤率为48%,死亡率为37%,至18周时才出现颊黏膜高分化鳞癌。结论经筛选采用0.3%DMBA丙酮液注射法可诱发形成口腔鳞癌动物模型,缩短建模周期,降低建模成本,能为科学研究提供较为理想的动物模型。

Objective To establish hamster buckle pouch carcinogenesis model with injection of DMBA acetone solution at different concentrations,and compares it with the traditional paint method.Methods Eighty one golden hamsters were assigned randomly into three groups： 60 hamsters in the injection groups were injected with DMBA acetone solution according at the concentrations of 0.5%,0.4%,0.3%,0.2%,0.1% respectively,twice a week,for 9 weeks.15 hamsters as the paint group were painted with 0.5% DMBA acetone solution,and 6 hamsters without any treatment belonged to the control group.General observation,weight,histopathology were executed every week.Three animals in every group were sacrificed by anesthesia at 3,6,9,12,18 weeks respectively and subjected to histological examination.Results The injection group with the concentration of 0.4%,0.3%,0.2%,0.1% appeared about 0.8 cm × 0.6 cm × 0.5 cm size of the tumor in 13,9,12,16 weeks respectively.The rate of carcinoma formation were 25%、87%、42% and 58% at 12 weeks.The mortality rate were 48%,11%,13% and 10% respectively.In the paint group tumor occurred in 18 weeks.The rate of carcinoma formation and mortality were 48% and 37% at 12 weeks.Conclusion The DMBA acetone solution at the concentration of 0.3% is more suitable for the formation of oral squamous cell carcinoma in golden hamster in the short term.This method is benefitial to shorten the feeding cycle,reducing the costs of the model.And it may provide an ideal animal model in vivo for further study.