microRNA-31在原发性肺癌组织中的表达及临床意义

Expression and Clinical Significance of microRNA-31 in Primary Lung Cancer Tumor Tissues

目的检测肺癌组织及癌旁正常组织中miR-31的表达情况与肺癌的诊断、肿块大小、淋巴结转移、吸烟及分期的关系。方法采用quantitative real-time RT-PCR方法对75例肺癌患者术后癌组织及癌旁正常组织中miR-31的表达量进行检测。结果 miR-31在癌组织中的相对表达量是癌旁正常组织的6.48倍(P<0.001)。吸烟患者miR-31的表达量是不吸烟患者的3.06倍(P=0.033)。在NSCLC中,与肿瘤长径≤3 cm肿瘤相比,长径>3 cm肿瘤中miR-31的表达量较高(P=0.045)。无论淋巴结转移与否,其表达量差异无统计学意义(P=0.703)。结论 miR-31在肺癌组织中高表达、在不同肿块中表达的差异以及与吸烟的关系,提示其可能成为肺癌的筛查、诊断及预测肿块大小的一个生物标志物。

Objective Detected the miR-31 expresssion level in tumor tissues and adjacent normal tissues to observe the relationship between miR-31 and the screening diagnosis of lung cancer.Methods Using quantitative real-time RT-PCR to assess the miR-31 expression in lung cancer tumor tissues and adjacent normal tissues.Results The relative expression level of miR-31 is 6.48 folds in tumor tissues compared to adjacent normal tissues（P0.001）.The level in smoke patients specimen is 3.06 folds to non-smoke.The expression level of tumor major axis3 cm is higher than major axis≤3 cm in NSCLC（P=0.033）.Whether the lymph nodes metastasis or not,there is no difference between them.Conclusion The outcomes that miR-31 was overexpressed in lung cancer,and the difference in accordance with tumor major axis as well as the relationship with cigarettes indicated miR-31 might be a biomarker for screening,diagnosis,and predicting the tumor axis.