去甲斑蝥素对Burkitt淋巴瘤Raji细胞株生物学特性的影响

Norcantharidin inhibits proliferation of Burkitt lymphoma Raji cells and induces cell cycle arrest

目的:探讨去甲斑蝥素(nor-cantharidin,NCTD)对Raji细胞的增殖抑制及周期阻滞作用。方法:通过台盼蓝拒染法观察NCTD对Raji细胞及外周血单个核细胞(perip heralblood mononuclear cells,PBMC)的增殖抑制情况;甲基纤维素集落形成法观察NCTD对Raji细胞自我更新能力的影响;流式细胞仪检测Raji细胞周期变化。结果:NCTD抑制Raji细胞增殖,具有剂量时间依赖性(P<0.05),但是对单个核细胞增殖无影响(P>0.05);NCTD显著抑制Raji细胞的集落形成能力(P<0.05),NCTD使Raji细胞G2/M期细胞增多,G0/G1期细胞和S期细胞减少。结论:去甲斑蝥素能抑制Raji细胞增殖并产生G2/M期阻滞,且对Raji原始干祖细胞有抑制作用,有可能成为一种新的抗瘤制剂作用于人Burkitt淋巴瘤。

Objective To investigate the effects of Norcantharidin （NCTD） on human Burkitt lymphoma cell line Raji proliferation and cell cycle arrest in vitro. Methods Trypan blue assay was used to detect the inhibiting rate of NCTD on Raji cells and peripheral blood mononuclear cells （PBMC）, in which concentration of NCTD were different. The self-renewal and.proliferating ability were examined by methylcellblose conlony-forming units （CFU） assay. The cell cycle were detected by flow cytometry. Results By using Trypan blue assay, we found that NCTD can inhibit the proliferation of Raji cells significantly in dose- and time-dependant manners （P 〈 0.05）, whereas the effect on PBMC was not significant （P 〉 0.05）. The CFU assay shows that the amount of colony-forming number decreased more in Raji cells administated with NCTD than in Raji cells which were pre-treated with NCTD for 48 h. The proportion of cells at the GJM stage increased while ones at the G0/Gl and S stage decreased. Conclusions NCTD inhibits the proliferation of Raji cells and induces cell cycle arrest at G2/M stage, and can strongly inhibit the growth of original Raji cells. NCTD may be useful for developing new therapeutic regimens for the treatment of human Burkitt lymphoma leukemia.