长期吸入低浓度七氟醚对雌性小鼠排卵的影响

Effects of long-term exposure to low concentration sevoflurane on ovulative function in female mice

目的:评价长期吸入低浓度七氟醚对雌性小鼠排卵功能的影响。方法:雌性昆明种小鼠40只,6周龄,体重(20±2)g,随机分为5组(n=8):实验组(ABCD组)分别吸入0.003%、0.03%、0.06%、0.1%七氟醚,每天6h,E组吸空气。于30d后,孕马血清促性腺激素(PMSG)、人体绒膜促性腺激素(HCG)促超排卵后处死动物,压片法计数卵母细胞,光学显微镜观察卵母细胞形态学。结果:与对照组相比,ABCD四个实验组小鼠卵母细胞数相近(P>0.05)、形态相似。结论:长期吸入≤0.1%七氟醚后,小鼠排卵功能未见明显影响。

Objective To evaluate effects of long-term exposure to low concentration sevoflurane on ovulative function in female mice. Method Forty female KM mice, aged 6 weeks, weighting 20 ＋ 2 g, were randomly divided into 5 groups （n = 8 each）： Mice in group A,B,C,D exposured respectively 0.003%, 0.03%, 0.06%, 0.1% sevoflurane 6h per day while mice in group E exposured air for 30d.Executed mice after superovulation which was used in all female mice to get more eggs at the end point of examination by using pregnant mare serum gonadotropin （PMSG） and human chorionic gonadotropin （HCG） Compressor method was used to count and observe. Results Four experimental groups A,B, C, D compared with the control group E did not have significant difference （P 〉 0.05） on the mice oocytes population, and plesiomorphism. Conclusion Long-term exposure to O. 1% or less sevoflurane do not lead to obvious influence in ovulation function in female mice by using PMSG and hCG.