耐碳青霉烯类大肠埃希菌与肺炎克雷伯菌的聚合酶链反应分析

Carbapenems-resistant genes in Escherichia coliand Klebsiella pneumoniae: analysis of polymerase chain reaction

目的了解耐碳青霉烯类抗菌药物大肠埃希菌和肺炎克雷伯菌的耐药基因,并通过测序确定耐药基因的碱基序列。方法 36株菌株进行MIC测定,筛选耐碳青霉烯类的多药耐药细菌;用聚合酶链反应(PCR)扩增耐碳青霉烯类菌株的基因,以确定基因的种类。结果 8株大肠埃希菌中4株有KPC基因,阳性率为50.0%;28株肺炎克雷伯菌中26株出现阳性菌株,阳性率为92.8%;36株中未发现VIM和IMP基因。结论宁波地区这几所医院中,大肠埃希菌和肺炎克雷伯菌中最主要是KPC基因,但是不能排除VIM和IMP基因。

OBJECTIVE To investigate drug resistance of carbapenems in Escherichia coli and Klebsiella pneumoniae and the correlative resistance genes and to identify the base sequences by sequencing.METHODS A total of 36 multidrug-resistant bacteria of carbapenems-resistant E.coli and K.pneumoniae were determined with MIC;the polymerase chain reaction（PCR） was carried out to amplity carbapenem-resistant genes,the PCR products were sequenced to identify the types of genes.RESULTS There were 4 strains with KPC gene among 8 strains of E.coli with the positive rate of 50.0%;26 positive strains were found among the 28 strains of K.pneumoniae with the positive rate of 92.8%.36 strains of E.coli and K.pneumoniae were not found for VIM and IMP genes.CONCLUSION Among several hospitals in Ningbo,the main KPC genes were in E.coli and K.pneumoniae,but the VIM and IMP genes cannot be ruled out.