黄瓜抗白粉病染色体片段导入系的SSR鉴定

Analysis of Cucumber Chromosome Segment Introgression Line with Powdery Mildew Resistance Based on SSR Markers

以黄瓜高抗白粉病品种JIN5-508为供体亲本,以高感白粉病品种D8为轮回亲本,通过多代回交后自交,并在每个世代结合白粉病抗性进行接种鉴定,获得了17个以D8为遗传背景但具有抗白粉病的染色体片段导入系。利用平均分布于黄瓜7条染色体上的324对SSR引物对17个导入系63个抗病单株（病情指数≤10）的染色体导入片段特征进行了鉴定。结果表明：74对SSR引物在亲本间表现多态性;63个抗病单株中单片段导入系共25株,占39.68%;导入双片段的共32株,占50.79%;有3个供体片段导入的为4株,占6.35%;无片段导入的2株,占3.17%。出现频率较高的导入片段共4个,分别位于1号染色体的23.4~41.3cM、3号染色体的51.1~54.0cM、4号染色体的9.3~11.3cM和6号染色体的1.4~3.2cM处,出现的频率分别为74.6%、44.4%、25.4%和7.9%。

17 cucumber chromosome segment introgression lines（CSILs）were constructed from a cross between the powdery mildew susceptible cultivar（D8）and resistant cultivar（JIN5-508）,and 63 plants with high powdery mildew resistance（disease index ≤ 10）were selected. In this study,324 pairs of SSR primers were applied to analysis the polymorphic level between JIN5-508 and D8,and 91 pairs of SSR primer which generated polymorphic bands were applied to analysis the distribution,segment size of the chromosome segments. The results indicated that 25 CSILs were single segment introgression line, which accounted for 39.68% of the total CSILs;Most of the CSILs were double segment introgression lines,take up to 50.79%;Four CSILs were imported 3 chromosome segments;No donor introgression segments were found in 2 lines. 4 donor segments occupied the highest frequency,which located on 23.4–41.3 cM of chromosome 1,51.1–54.0 cM of chromosome 3,9.3–11.3 cM of chromosome 4 and 1.4–3.2 cM of chromosome 6,respectively.