麝香百合花发育基因LLGLO1在花蕾中的时空表达

The Temporal and Spatial Changes of Flower Development Gene LLGLO1 in the Floral Buds of Lilium longiflorum

为了获得百合花发育相关的B功能基因LLGLO1在调控百合花发育过程中的重要信息,以麝香百合(Lilium longiflorum)未开放的花蕾为试材,以LLGLO1基因的特异片段为探针,应用RNA原位杂交技术,对LLGLO1基因在花发育各个阶段的时空表达动态进行了研究。结果表明,LLGLO1基因在外轮花被、内轮花被、雄蕊和心皮等4轮花器官中均有表达,但表达强度随不同的发育阶段而变化。长度2.0和3.0cm的花蕾中LLGLO1基因的表达量明显高于0.5和1.0cm的花蕾,说明随着花器官的发育成熟,该基因在各轮花器官中的表达量逐渐增加。

In order to obtain the important expression information of LLGLO1 gene during the process of flower development,the temporal and spatial changes of LLGLO1 gene at different development stages of floral buds by in situ hybridization,using the floral buds of Lilium longiflorum as materials and the special sequence of LLGLO1 gene as the probe. Result indicated that LLGLO1 gene was expressed in the four whorls of floral-organ,including outer tepal,inner tepal,stamen,and carpel. It also indicated that the expression level of LLGLO1 gene varies with different development stages of floral buds. The expression level was higher in the 2.0 cm and 3.0 cm floral buds than that in the 0.5 cm and 1.0 cm ones,showing that the expression level of LLGLO1 gene increases with the development of floral-organ.