1，2-二氯乙烷对体外培养SW620细胞增殖和细胞周期及凋亡的影响

The effects of 1, 2-dichloroethane on the cellular proliferation, cellular cycle and apoptosis of SW620cells in vitro

目的探讨1，2．二氯乙烷（1，2-DCE）对体外培养细胞周期、凋亡和增殖能力的影响。方法不同浓度1，2-DCE染毒0．5或1h，噻唑蓝（MTT）法检测活细胞相对数和相对活力，流式细胞术（FCM）分析细胞周期和凋亡情况。结果MTF法检测发现，随1，2-DCE染毒剂量的增加和染毒时间的延长，细胞相对存活率逐渐降低。与二甲亚砜（DMSO）组比较，染毒0．5h，25、75、100、125、150、175、200μmol／L1，2-DCE组的细胞相对存活率降低，差异均有统计学意义（P〈0．05或P〈0．01）；染毒1h，75、100、125、150、175、200μmol／L1，2-DCE组的细胞相对存活率降低，差异均有统计学意义（P〈0．05或P〈0．01）；与0．5h各组比，175μmol／L组染毒1h的细胞相对存活率降低。差异有统计学意义（P〈0．01）；增殖曲线经标准化拟合后发现，染毒0．5h，1，2-DCE的最适Ic。为89．41μmol／L，95％的可信区间为85．23-93．79μmol／L；染毒1h，1，2-DCE的最适Ic50为87．68μmol／L，95％的可信区间为83．71-91．82μmol／L。FCM法检测发现，与对照组比较，1，2-DCE染毒1h，25、50、100、150、200μmol／L组的G0/G1期比例降低，25、50、100μmol／L组的S期比例降低，25、50、100、150、200μmol／L组的GJM期比例升高，差异均有统计学意义（P〈0．05或P〈0．01）；但各组均不引起细胞凋亡。结论1，2-DCE能够抑制体外培养SW620细胞增殖，使细胞周期停滞在GJM期，但不诱导细胞凋亡。

Objective To explore the effects of 1, 2-dichloroethane （1, 2-DCE） on the cellular proliferation, cellular cycle and apoptosis of SW620 cells in vitro. Methods SW620 cells were exposed to 1,2- DCE at different concentrations for 0.5 and 1 h. MTT assay was used to detect the relative number and relative viability, the low cytometry （FCM） assay was utilized to measure the cell cycle and apoptosis. Results The results of MTF assay showed that the cellular relative viability decreased with the 1, 2-DCE＇ s dose and exposure time. Compared with the DMSO group, the relative cellular viability of ceils exposed to 1, 2-DCE at the doses of 75, 100, 125, 150, 175, 200 μmol/L for 1 h decreased （P〈0.05 or P〈0.01）. Compared with the grouos exposed to 1,2-DCE for 0.5 h, the relative cellular viability of cells exposed to 175μmol/L 1,2-DCE for 1 h decreased significantly （P〈0.01）. IC50 of cellular proliferation in cells exposed to 1,2-DCE for 0.5 h was 89.41 txmol/L, and 95% confidence interval was 85.23 to 93.79 μmol/L. IC50 of cellular proliferation in cells exposed to 1,2-DCE for 1 h was 87.68μmol/L, and 95% confidence interval was 83.71 to 91.82μmol/L. The results of FCM indicated that compared with the control group, the G0/G1 phase in groups exposed to 1, 2-DCE at the doses of 25, 50, 100, 150 and 200μmol/L for 1 h decreased significantly （P〈0.05 or P〈0.01 ）, the S phase in groups exposed to 1, 2-DCE at the doses of 25, 50 and 100μmol/L for 1 h reduced significantly （P〈0.05 or P〈0.01）, the G2/M phase in groups exposed to 1, 2-DCE at the doses of 25, 50, 1190, 150 and 200μmo//L for 1 h increased significantly （P〈O.05 or P〈0.01）. However, 1, 2-DCE could not induce apoptosis of SW620 cells. Conclusion 1, 2-DCE could inhibit the proliferation of SW620 cells, and arrest SW620 cells at G2/M phase, but could not induce the apoptosis of SW620 cells in vitro.