蚕豆种子贮藏蛋白质组分的比较研究

Comparative Study on Seed Storage Protein of Faba Bean

以10份蚕豆品种为材料,利用SDS-PAGE方法,对其种子贮藏蛋白亚基组成进行了分析,结果表明:不同品种间的种子贮藏蛋白具有一定的差异,表现出一定的多态性;共分离出23条迁移率不同的亚基条带,13条具有多态性;利用贮藏蛋白亚基条带的信息,分析了品种间的蛋白相似度,相似度指数0.619～0.947,平均0.744;并通过聚类分析,在遗传相似系数0.8260水平上,将供试材料分为了3类,其中第2类具有较丰富的多样性,多样性指数达3.9296;共筛选出7对亲缘关系较远的杂交组合,可为蚕豆品种选育提供一定的科学依据。

In order to investigate the difference of seed storage proteins in Faba bean, seed storage proteins ex- tracted from ten species of Faba bean were analyzed with SDS-PAGE. The result showed that it is different among the seed storage proteins of faba bean, a total of 23 subunits of different Rf were detected, counting 13 polymorphic protein subunits. The similarity index of seed storage proteins subunits in Faba bean is 0. 619 -0. 947, with an av- erage of 0. 744. Cluster analysis divided the accessions into three major clusters in the level of 0. 8260 GS, the sec- ond cluster showed higher polymorphism, and 7 pair Faba bean were identified as the parents.