摘要
利用SSR标记研究了22份薏苡种质的遗传多样性,用11对扩增带型稳定的SSR引物从供试材料中检测出105个等位基因变异,每对引物检测等位基因4～20个,平均9.55个。SSR引物的PIC介于0.3048～0.9238,平均多态性信息量为0.8255。利用UPGMA聚类分系法将供试自交系划分为4类,该划分结果与根据地理来源、种质系谱的分类结果基本一致。SSR分子标记辅助的种质改良是薏苡品种改良的重要途径。
The genetic diversity of 22 Jobs tears germplasm were studied by simple sequence repeats （SSR） markers. 11 SSR primers giving stable amplified band pattern detected 105 alleles among the lines tested. The av- erage number of alleles per SSR locus were 9.55 with a range from g to 20. The value of polymorphism information content （PIC） for each SSR locus varied from 0. 3048 to 0. 9238 with average of 0. 8255. The 22 germplasm were divided into 4 groups by UPGMA method based on SSR fingerprinting. The clustered results were similar to that based on geograph-ical resource and germplasmic genealogy. SSR marker could be an accurate and reliable method to study diversity of Jobs tears germplasm. SSR marker assisted genetic improvement of Jobs tears germplasm would be an important way to breed new Jobs tears cuhivars.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2012年第2期317-320,共4页
Journal of Plant Genetic Resources
基金
贵州省年度攻关项目(黔科合NY字[2008]3034号)
关键词
薏苡
种质
遗传多样性
SSR
Jobs tears
Germplasm
Genetic diversity
SSR
