甘蔗野生种滇蔗茅种质创新利用研究Ⅰ.甘蔗与滇蔗茅远缘杂交F_1群体构建与SSR分子标记鉴定

Innovation Germplasm of Erianthus rockii Ⅰ. Establishment of F_1 Intergeneric Distant Hybridization of Saccharumofficinarum L. and Erianthus rockii and the Identification of F_1 by SSR Molecular Markers

利用光周期调控技术诱导甘蔗热带种路打士开花,并与滇蔗茅云南95-19进行远缘杂交,培育实生苗76株,大田移栽成活62株,成活率81.6%;选用4对引物对获得的62份杂交后代进行SSR分子标记鉴定,结果表明,62份杂交后代全部为真杂种,所选SSR引物对滇蔗茅后代群体具有较高的鉴定效率。该杂交组合后代杂种真实率为100%,是目前数量最大的滇蔗茅F1群体。

Using photoperiod pathway controlling techniques induced S. officinarum Ludashi to bloom, and dis- tant crossing with Erianthus rockii Yunnan 95-19. 62 seedlings have been transplanted and survived in the field. The survival rate was 81.6%. 4 pairs of SSR primers were chosen to use for authenticity identification of progenies. The results showed that all of the 62 progenies were genuine hybrids, and these SSR primers are quite efficient for identification of Erianthus rockii progenies. The genuine hybrids rate of this cross was 100% , and the F1 population of Erianthus rockii progenies was the biggest one at present.