冷藏鲤鱼和罗非鱼优势腐败菌腐败能力分析

Analysis of Spoilage Ability of Dominant Spoilage Bacteria from Stored Chilled Cyprinus carpio and Oreochromis niloticus

通过分析接种腐败菌的鲤鱼和罗非鱼无菌鱼块贮藏中感官、腐败代谢产物和腐败菌的变化,以腐败菌的生长动力学参数和腐败代谢产物的产量因子(YTVBN/CFU)为指标,探讨冷藏鲤鱼和罗非鱼优势腐败菌假单胞菌和腐败希瓦氏菌的腐败能力。结果表明:接种腐败希瓦氏菌和恶臭假单胞菌的鲤鱼无菌鱼块的货架期分别为132h和162h,此时的TVBN值为27.12mg/100g和22.51mg/100g,腐败希瓦氏菌和恶臭假单胞菌菌数为8.96 lg(CFU/g)和9.07 lg(CFU/g),产量因子YTVBN/CFU为9.28×10-9mg TVBN/CFU和1.81×10-8mg TVBN/CFU。接种荧光假单胞菌和腐败希瓦氏菌的罗非鱼无菌鱼块的货架期分别为132h和144h,此时的TVBN值为23.46mg/100g和24.30mg/100g,荧光假单胞菌和腐败希瓦氏菌菌数为8.83 lg(CFU/g)和9.12 lg(CFU/g),产量因子YTVBN/CFU为1.67×10-8mg TVBN/CFU和9.10×10-9mg TVBN/CFU。结合两种养殖鱼冷藏过程中的菌相变化和腐败菌在腐败过程中的作用,初步得出冷藏罗非鱼和鲤鱼的特定腐败菌是假单胞菌,两种腐败菌都具有较强的腐败能力。

Sensory quality, the growth kinetic parameters of spoilage bacteria and the yield factor of metabolic products, YTVBN/CFU, were assayed on sterile Cyprinus carpio and Oreochromis niloticus tissue blocks inoculated with spoilage bacteria during chilled storage. The spoilage ability of two dominant spoilage bacteria, Pseudomonas spp. and Shewanella putrefaciens, were analyzed on Cyprinus carpio and Oreochromis niloticus stored aerobically under chilled conditions. The post-inoculation shelf-lives of sterile Cyprinus carpio tissue blocks inoculated with Shewanella putrefaciens and Pseudomonas putida were 132 h and 162 h, respectively, the TVBNs 27.12 mg/100 g and 22.51 mg/100 g, the bacterial counts 8.96 lg（CFU/g） and 9.07 lg（CFU/ g） and the YTWBN/CFU values 9.28×10^-9mg TVBN/CFU and 1.81 ×10^-8mg TVBN/CFU. The post-inoculation shelf-lives of sterile Oreochromis niloticus tissue blocks inoculated with Pseudomonasfluorescens and Shewanella putrefaciens were 132 h and 144 h, respectively, the TVBNs 23.46 mg/100 g and 24.30 mg/100 g, the bacterial counts 8.83 Ig（CFU/g） and 9.12 lg（CFU/g） and the YTVBN/CFU values 1.67 × 10^-8 mg TVBN/CFU and 9.10 × 10^-9 rng TVBN/CFU. Based on the microflora changes and the role of the spoilage bacteria during chilled storage of the two species of cultured fish, it can be preliminarily concluded that the special spoilage organisms of stored chilled Oreochromis niloticus and Cyprinus carpio was Pseudomonas spp and that the spoilage ability of Pseudomonas spp and Shewanella putrefaciens is strong.