超滤技术在蛋白质分离纯化中的应用

Application of Ultrafiltration Technique in Protein Separation and Purification

超滤技术是具有效率高、能耗低、无相变、无需添加任何化学试剂、操作简便、条件温和等优点的一种膜分离技术,其用于蛋白质分离纯化具独特优势。综述超滤技术在蛋白质脱盐、脱醇、浓缩、分级分离和内毒素去除等过程中的应用研究,并探讨该项技术应用中面临的问题与解决方法。

Ultrafiltration is a membrane separation technique, which shows unique advantages, such as high efficiency, low energy consumption, no phase change, no use of chemicals, simple and easy operation and mild condition, in protein separation and purification. The researches on application of uhrafiltration technique in protein desalination, de-alcoholizing, concentration, fractionation and endotoxin removal were reviewed and the existing problems in the application and their solutions were also discussed.