Klebsiella pneumoniae在不同发酵罐中发酵甘油制1,3-丙二醇的控制条件研究

Fermentation Conditions of Glycerol for Production to 1,3-Propanediol by Klebsiella pneumoniae in Different Reactors

对克雷伯氏肺炎杆菌间歇发酵甘油产生1,3-丙二醇的发酵条件进行了优化,确定了50L搅拌式发酵罐和15L气升式发酵罐发酵的最佳发酵条件,得到的1,3-丙二醇的浓度、生产率和甘油摩尔转化率分别是80.97g/L、1.69 g/(h·L)、0.57 mol/mol和74.81 g/L、1.56 g/(h·L)、0.50 mol/mol;对比了不同类型发酵罐的发酵结果,表明50 L搅拌式发酵罐发酵结果优于15L气升式发酵罐,前者相对于后者来说,1,3-丙二醇的浓度和甘油摩尔转化率分别提高了8.23%和14.91%;确定了最适底物浓度(以初始甘油浓度计)在25 g/L左右。

The conditions under which Klebsiella pneumoniae batch fermentation glycerol produced 1,3-propane- diol（PDO） were optimized in this study; thereby the best fermentation conditions for the STR-50L and the ATR-15L were determined and the ultimate consistency and productivity rates of PDO and the molar yield of PDO from glycerol were 80.97 g/L, 1.69 g/（h·L）, 0.57 mol/mol, 74.81 g/L,1.56 g/（h·L） and 0.50 mol/mol, respectively. In comparison with the latter, the concentration of PDO and glycerol mole conversion of the former was increased by 8. 23% and 14.91% , respectively. Furthermore, the most appropriate substrate concentration was approximately 25g/ L（ in the initial glycerol concentration of total）.