固相萃取-高效液相色谱法测定啤酒原辅料中黄曲霉毒素B_1

Determination of Aflatoxin B_1 in Raw Materials of Beer by SPE-HPLC

以啤酒酿造中主要原辅料(大麦麦芽、大麦、大米、小麦麦芽)为样品,比较了几种不同净化柱净化黄曲霉毒素B1(AFB1)的回收率,最终选择了硅胶-硅藻土-中性氧化铝混合柱作为净化柱,建立了一种检测啤酒原辅料中AFB1的反相高效液相色谱(RP-HPLC)方法。结果表明,AFB1的检测线性范围为0.5～50μg/kg,线性相关系数r为0.999 7。方法检出限为0.15μg/kg,加标回收率80.1%～109.2%,相对标准偏差(RSD)为0.23%～4.29%。该方法简便、准确、成本低廉,可用于啤酒原辅料中的AFB1的质量控制。

By comparing the recoveries of different solid phase extraction columns for aflatoxin Bl （ AFB1 ） clean- up, the silicone-diatomite-neutral alumina mixed column was chosen, and the analytical method of Reversed Phase High Performance Liquid Chromatography （RP-HPLC） was developed for determination of aflatoxin B1 in raw materi- als of beer （barley malt, barley, rice and wheat malt）. The results showed that the linear range of detection of AFBt ranged from 0.5μg/kg to 50 μg/kg. Standard curve correlation coefficient（r） was 0. 9997. The detection limit was 0. 15μg/kg. The average recovery ranged from 80. 1% to 109.2%, and the relative standard deviation（RSD） ranged from 0.23% to 4.29%. The method was simple, accurate and cost saving. It was also proposed to be used for the quality control of AFB1 in raw materials of beer.