PCR检测急·慢性弓形虫感染小鼠的研究

Detection of Toxoplasma gondii by Polymerase Chain Reaction(PCR) in Acute and Chronic Infected Mice

[目的]建立灵敏、特异、稳定的检测弓形虫感染的PCR法。[方法]检测弓形虫急、慢性感染鼠血清及组织中弓形虫速殖子DNA,并对检测感染鼠血清中弓形虫速殖子的灵敏度进行评估。[结果]急性感染鼠接种后18 h,40%的感染鼠被检测为阳性;接种后21 h,80%的感染鼠被检测为阳性;24 h以后100%的感染鼠被检测为阳性。慢性感染鼠接种后18 d可从肝、肺、脾、肾、脑组织中检测到弓形虫DNA。试验中检测到1个弓形虫,相当于0.2 pg的弓形虫DNA含量。[结论]结果为运用PCR法检测弓形虫感染提供了参考。

[ Objective] Tbe aim was to establish a high sensitive,specific and stable PCR technique for detecting T. gondii. [ Methods] Detecting T. gondii in acute and chronic infected mice and evaluating the sensitivity of detection of T. gondii DNA in sera of acute infected mice. [ Result] After inoculating for 18,21 and 24 h of acute infected mice,40% ,80% and 100% ,respectively infected mice were detected as positive. DNA of T. gondii could be detected in liver,spleen,lung,kidney and brain after inoculating for 18 d. In the test,we detected that one T. gondii was equal to DNA content of 0.2n~ T. uondii. V Canclusion] The results t）rovide reference for the detection of T. ~orldii in mice by PCR.