鸡传染性支气管炎病毒的分离与鉴定

solation and Identification of Avian Infectious Bronchitis Virus

[目的]从发病鸡群中分离出鸡传染性支气管炎病毒,并对其进行鉴定。[方法]从安徽某鸡场发病鸡群中分离出鸡传染性支气管炎病毒,采用鸡胚盲传,观察病毒对鸡胚的致病作用。通过动物回归试验,在SPF鸡上复制出支气管堵塞的症状,扩增分离毒株的S1基因片段,并与IBV疫苗毒株进行比较。[结果]对分离到的毒株进行HA检测,结果表明收获的尿囊液对鸡红细胞无凝集活性,说明分离到的病毒中无NDV、AIV等,但经1%胰酶处理则可凝集鸡红细胞,符合传染性支气管炎病毒的生物学特征。该毒株的第6代SPF鸡胚尿囊液通过滴鼻接种SPF鸡,可复制出与现场相似的临床症状,其发生了支气管堵塞的现象,初步证实分离的病毒为一株鸡传染性支气管炎病毒,命名为XZ株。[结论]该研究为鸡传染性支气管炎的防治提供了理论依据。

[ Objective ] The aim of the study was to isolate an avian infectious bronchitis virus （IBV） isolate from the diseased chickens and iden- tify its characteristics. [ Method] An IBV isolate was isolated from the diseased chickens in a chicken farm in Anhui Province and passaged blindly in chicken embryos to observe its pathogenicity. Then animal regression test was used to replicate bronchial congestion in SPF chickens. The S1 gene fragment was amplified and its sequence was aligned with the IBV vaccine strains. [ Result ] The results of HA assay showed that the allantoie fluid of the IBV strain had no agglutination activity toward chicken red blood cells. This result suggested that no Newcastle disease virus and avian influenza virus were found in the allantoic fluid. However, the allantoic fluid treated with 10 g/L trypsin solution could agglutinate chicken red blood cells,which is in line with the biological characteristics of IBV. After the SPF chickens were inoculated with the sixth passage of the IBV i- solate ,the clinical symptoms like bronchial congestion that were similar with those in clinic were observed. The results initially confirmed the iso- lation of an IBV isolate,which was named IBV XZ strain. [ Conclusion ] This study provides a theoretical basis for the prevention and treatment of avian infectious bronchitis.