马立克氏病鸡MDV血清基因的克隆与鉴定

Cloning and Identification of L-meq Gene of Marek's Disease Virus

[目的]克隆马立克氏病鸡MDV血清基因,并对其进行鉴定。[方法]从潜伏期感染马立克氏病鸡淋巴组织中提取基因组DNA,采用梯度PCR扩增马立克氏病毒的L-meq基因。将其插入pMD18-T克隆载体并进行测序,并用DNAman软件进行序列分析。[结果]获得的DNA片段的序列与已发表的L-meq基因序列相一致,表明该试验成功获得了目的基因。[结论]该研究可为鸡马立克氏病的预防与治疗提供新思路和新方法。

[Objectlve]To clone and identify Marek s thsease v,rus （MDV） serum gene. [Method] MDV genomic DNA was extracted from lymphoid tissues of the diseased chickens with latent MDV infection. The MDV L-meq gene was amplified by gradient PCR,inserted into pMD18 t ： -T vector and sequenced. The sequence was analy^d using DNAman software. [ Result] The obtained sequence had 100% similarity with the ,published sequence of L-meq gene ,showing successful amplification of target gene. [ Conclusion] The paper provides new ideas and new methods for prevention and treatment of Marek＇ s disease in chickens.