摘要
[目的]研究红花苷对K562白血病细胞的抑制增殖和诱导分化作用。[方法]以培养的K562白血病细胞为试验对象,通过XTT试验、集落形成试验、血红蛋白联苯胺染色试验以及形态学试验,考察红花苷对白血病细胞抑制增殖和诱导分化作用。[结果]红花苷剂量依赖性地抑制了K562白血病细胞的活性及集落形成(P<0.05),形态学观察及联苯胺染色试验也证实了红花苷剂量依赖性地诱导K562白血病细胞向产生血红蛋白的终末细胞分化。[结论]红花苷对K562白血病细胞具有明显的抑制增殖和诱导分化作用。
[ Objective ] To research the proliferation inhibition and induced differentiation effects of carthamin on K562 leukemic cells. [ Method] With the cultured K562 leukemic cells as the research materials, the proliferation inhibition and induced differentiation effects of carthamin were observed by the XTI＂ test, colony formation test, hemoglobin benzidine staining test and morphological experiment. [ Result ] Carthamin dose dependently （10-5 mol/L, 10-4 mol/L, 10-3 mol/L） inhibited the cytoactive and colony formation （P 〈 0.05 ） ; the hemoglobin benzidine staining test and morphological experiment also proved that the carthamin dose dependently induced the K562 leukemic cells to the hemoglobin end ceils. [ Conclusion] Carthamin had significant effects of proliferation inhibition and induced differentiation on K562 leukemic cells.
出处
《安徽农业科学》
CAS
2012年第9期5165-5166,5233,共3页
Journal of Anhui Agricultural Sciences
基金
河南省教育厅2010年度自然科学研究计划项目(2010B320011)
