不同基原的郁金类药材中郁金多糖的含量测定

Determination of Polysaccharide Contents in the Different Base Resources of RADIX CURCUMAE

[目的]研究不同基原的郁金类药材中郁金多糖的含量,为郁金类药材的质量控制提供依据。[方法]采用热水浸提法提取郁金粗多糖,经过纯化得到郁金多糖,然后采用分光光度法,经苯酚-硫酸显色后,在489 nm波长处测定其总糖、还原糖及多糖的含量。[结果]不同基原的郁金中多糖的含量不同;3个不同基原的郁金药材中,以绿丝郁金多糖含量最大,其多糖的平均含量为25.775%;桂郁金的次之,其多糖含量为3.955%;黄丝郁金的多糖含量最低,为2.695%。[结论]该方法操作简单,稳定性好,可用于郁金中多糖的含量测定。

[ Objective] To study the polysaccharide contents in different base recourses of＂ RADIX CURCUMAE, and to provide references for the quality control of RADIX CURCUMAE. [ Method ] The crude polysaecharide was extracted by hot water extraction ; after using the spectrophotometry and phenol-sulfuric method, the contents of total sugar, reducing sugar and polysaccharide in the purified polysaccharide were detected at 489 nm. [ Result] Polysaccharide content varied in different base recourses of RADIX CURCUMAE. Among the 3 different base resources of RADIX CURCUMAE, Curcuma phaeocau lis Val. had the maximum polysacchafide content （25. 775% ）, followed with Cur- cuma kwangsiensis S. G.. Lee et C. F. Liang （3. 955% ） ; and Curcuma longa. L. had the minimum polysaccharide content （2. 695% ）. [ Conelusionl This method was simple and stable and could be used for the content determination of polysaeeharide in RADIX CURCUMAE.