青蛤不同地理群体遗传结构差异的AFLP分析

AFLP Analysis of Genetic Structure of Cyclina sineusis.from Different Geographical Populations

[目的]探讨青蛤种群遗传多样性水平和遗传分化特征。[方法]采用AFLP技术对我国山东潍坊(WF)、江苏南通(NT)、浙江宁波(NB)、浙江温州(WZ)沿海共4个青蛤地理群体的遗传结构差异进行了分析。[结果]5对引物共得到261个位点,4个群体的多态位点比例平均为82.95%,其中WZ群体最高(86.97%),NT群体最低(78.93%);4个群体Nei’s基因多样性指数分别为0.258 1、0.252 6、0.271 3和0.277 6,香农多样性指数分别为0.394 7、0.383 0、0.412 0和0.421 4;群体遗传变异主要来自于群体内,占96.39%;WF和NT群体、NB和WZ群体遗传关系较近,聚类分析分别先聚在一起。[结论]研究的青蛤4个群体遗传基础较好,且尚未有明显的遗传分化。

[ Objective ] The aim was to discuss the genetic diversity level and classification of Cyclina sineusis. [ Method ] The genetic structure of four geographical populations from costal China of Weifang of Shandong （WF） , Nantong of Jiangsu （NT） , Ningbo of Zhejiang （NB） ,and Wenzhou of Zhejiang （WZ） were analyzed by using AFLP Markers. [ Result ] The total 261 clear amplification bands were detected through five pairs of AFLP primer, the average rate of polimorphic loci was 82.95%, of which the greatest was WZ population（ 86.97% ） and the lowest was NT population（78.93% ）. The gene diversity indexes of g populations of WF, NT, NB and WZ, the Nei were 0. 258 1, 0. 252 6, 0. 271 3 and 0. 277 6, and Shannon＇ s information index were 0. 394 7, 0. 383 0, 0. 412 0 and 0. 421 4. Genetic variations mainly came from individuals within populations （96.39%）. WF and NT population, NB and WZ population were clustered together because of close genetic relation. [ Conclusion] The genetic heredity of Cyclina sineusis, was good and it showed indistinct heredity classification.