摘要
[目的]了解自有YB菌株在BOD生物传感器及河豚毒素毒性传感器中的作用。[方法]以YB菌株作为响应菌株,采用夹层膜法制作固定化微生物膜,组装成微生物传感器并测试其性能。[结果]YB菌株在33℃,pH为7.2的条件下对BOD响应良好,在此条件下静态法测得传感器标准曲线为y=0.061 2x-0.001 3,线性相关系数为0.995;动态法测得传感器标准曲线为y=0.072 2x-0.031 9,线性相关系数为0.996。同时,YB菌株对河豚毒素基本没有响应。[结论]自有YB菌株可以作为BOD微生物传感器的敏感菌,但不能作为河豚毒素毒性传感器测试菌株。
[ Objective] The aim was to learn the function of YB strain in BOD biological sensor and toxin in dolphin. [Method] Considering YB strain as responding strain, and using membrane to fix microbiological membrane, the microbiological sensor was assembled and tested. E Result] When the determination of YB strain on BOD was at 33℃ and a pH of 7.2, its response to BOD was good. Under such condition, standard curve of sensor by static method was y =0. 061 2x -0. 001 3. Linear relevant coefficient was 0. 995. Standard curve of sensor by dy- namic method was y = 0. 072 2x - 0. 031 9. Linear relevant coefficient was 0.996. In the meantime, YB strain didn＇ t response to toxin in dol- phin. [ Conclusion] YB strain can be considered as BOD micro biological sensor. However. it can＇t test strain as toxin sensor of dolohin.
出处
《安徽农业科学》
CAS
2012年第9期5484-5486,共3页
Journal of Anhui Agricultural Sciences
基金
2011秦皇岛市科学技术研究与发展计划项目(201101A202)
