厚朴中厚朴酚及和厚朴酚的分离条件初探

The Exploration of the Separation Conditions for Magnolol and Honokiol in Magnoliae Cortex

目的:研究硅胶柱层析法分离纯化厚朴中厚朴酚及和厚朴酚的优化工艺。方法:采用不同体系的洗脱液进行洗脱。以厚朴酚的纯度为考察指标筛选最佳洗脱系统。结果:四种不同系统的不同比例均分离得到了含量大于90%的单个化合物晶体,其中优选的洗脱系统为石油醚∶丙酮系统,比例为10∶1,厚朴酚纯度为98.34%,收率96.43%。结论:四种洗脱系统中以石油醚∶丙酮系统效果最佳。

Objective：To study the silica gel column chromatography separation and purification in magnolia bark MagnoloI and Honokiol optimization of process. Methods ： Using different system of run in with fluid. To Honokiol purity for complete index se lects the optimal elution system. Result：Four kinds of different system of different scale to all separation got content than the 90 of the individual compounds crystals. One optimal elution system for petroleum ether： acetone system and the proportion is 10：1. Honokiol purity of 98.34 %and the yield was 96.43 ~. Conclusion：The best elution system is petroleum ether： acetone system.