牛ANGPTL2基因结构与功能的生物信息学分析

Bioinformatics Analysis of Bovine ANGPTL2 Gene Structure and Function

以牛的血管生成素样蛋白2(Angiopoietin-like protein2,ANGPTL2)基因为研究对象,运用生物信息学方法对其编码的蛋白质结构、理化性质及功能结构域进行分析。结果表明:牛ANGPTL2基因cDNA全长21 141 bp,最长开放阅读框为1 482 bp,编码493个氨基酸;序列比对结果显示,牛的ANGPTL2基因与人、家犬、鸭嘴兽、原鸡、斑马鱼、家马、小家鼠、野猪的核苷酸序列同源性分别为25.3%,24.7%,24.2%,24.3%,24.8%,24.9%,25.3%,25.5%;牛AN-GPTL2基因编码的氨基酸序列与上述其他动物的同源性分别为98.0%,96.8%,9.1%,6.3%,6.9%,51.9%,94.5%,98.2%;系统进化分析结果显示,在人、野猪、家犬、家马等动物中,牛的ANGPTL2基因与野猪的进化关系最为密切,亲缘关系最近;牛ANGPTL2蛋白中α螺旋较多,含有一个跨膜区。

Protein structures, physical and chemical properties, and their functional domain structure encoded by bovine angiopoietin-like protein2（ANGPTL2） gene were analyzed using bioinformatics method. Sequencing and bioin- formatics analysis showed that the bovine ANGPTL2 cDNA sequence was 21 141 base pairs long with an open reading frame（ORF）containing 1 482 nucleotides encoding a protein of 493 amino acids. The multiple sequence alignment showed that coding nucleotide sequences of the obtained bovine ANGPTL2 gene had 25. 3% , 24.7% , 24. 2% , 24.3% ,24.8% ,24.9% ,25.3% ,25.5% homology with the corresponding Homo sapiens, Canis lupus familiaris, Orni- thorhynchus anatinus, Callus gallus, Danio rerio, Equus caballus, Mus musculus, Sus scrofa, respectively, and the de- duced amino acid sequences had 98.0% ,96.8% ,9.1% ,6.3% ,6.9% ,51.9% ,94.5% ,98.2% identity with the cor- responding species respectively. Phylogenetic tree analysis of the amino acid sequences of bovine ANGPTL2 and other ANGPTL2 proteins in Homo sapiens ,Sus scrofa ,Canis familiaris ,Equus caballus and other animals showed that ,among all the species mentioned above,the nearest relationship exited between Bos taurus and Sus scrofa. Further analysis on the structure and function of the bovine ANGPTL2 protein showed that there were much more a helix than other see- ondary structure in the bovine ANGPTL2 protein,besides,it had a transmembrane region.