低温胁迫下不同磷营养对水稻叶片质膜透性及抗氧化酶活性的影响

Effects of Different Phosphate Fertilizer Application on Permeability of Membrane and Antioxidative Enzymes in Rice under Low Temperature Stress

采用盆栽试验的方法,以耐冷性品种吉粳81和非耐冷性品种长白9为供试材料,通过不同的磷肥施用量,研究低温胁迫下磷营养对水稻质膜透性及抗氧化酶活性的影响。结果表明:施磷后,随着施磷量的增加,2个水稻品种叶片电解质外渗率和丙二醛含量均先降低后升高,而可溶性糖、脯氨酸含量、过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的活性则呈先升后降的趋势。通过对比常温对照发现,低温下适当增磷可明显提高2水稻品种叶片可溶性糖、脯氨酸含量及3种抗氧化酶活性的上升幅度,减缓电解质外渗率和MDA含量的下降幅度。研究还表明,低温增磷对提升非耐冷品种长白9叶片可溶性糖、脯氨酸含量和3种抗氧化酶活性,降低电解质外渗率和丙二醛含量效果更明显。因此,通过体外增施磷肥可明显提高叶片质膜透性及抗氧化酶活性,从而有利于提高水稻的抗寒性能力。

A pot experiment was conducted to investigate the effects of different level of phosphate fertilizer ap- plication on permeability of membrane and antioxidative enzymes in rice（ non-chilling cv. Changbai 9 and chilling- tolerant cv. Jijing 81 ）under low temperature stress. The results showed that as the increasing of P fertilizer applica-tion, membrane permeability and MDA decreased and the increased in P-treated rice, but soluble sugar content, pro- line,peroxidase（POD） ,catalase（CAT） and superoxide dismutase（SOD） increased and then decreased. Compared with the normal temperature treatment,appropriately increasing phosphorus can improve the content of soluble sug-ar,praline,add the activity of POD,CAT, SOD,and decline membrane permeability and MDA content in rice under low temperture stress. The increase rates of the soluble sugar,proline,POD, CAT,SOD for P treatment in non-chill- ing cv. Changbai 9 were higher than those in chilling-tolerant cv. Jijing 81 under low temperature stress, and mem-brane permeability and MDA content decreased even more. The results indicated that membrane permeability and antioxidative enzymes of rice can be improved by exogenous application of phosphorus, it is beneficial to increase the cold resistance of rice.