新型高效降酚菌的鉴定及特性研究

Identification and Characteristics of New High Efficiency Phenol-degradation Strains

目的提高生物处理焦化废水效率,为工业废水处理用菌剂研究提供微生物资源。方法从某焦化废水厂的活性污泥中通过微生物方法筛选、分离,并用分子生物学方法对所得菌株进行鉴定。采用比浊法测定微生物量,研究菌株生长情况及降酚效果。结果得到三株降酚菌,均能在以苯酚为唯一碳源的培养基中生长,在72 h内将初始浓度800 mg/L的苯酚完全降解。对菌株的16s rDNA序列分析并与已发现的菌株进行同源性比对结果表明,三菌株分别属于喜盐芽孢杆菌属(Halobacillus sp.)、假单胞菌属(Pseudomonas sp.)和红球菌属(Rhodococcus sp.)。结论实验所得菌株为新型高效降酚菌,其降酚效果优异并可用于焦化废水处理。

Objective To improve the biological treatment efficiency of wastewater from coke-plant and to provide microbial resources for industrial wastewater treatment research. Methods The strains were separated and obtained from the activated sludge of coking wastewater plant by microbiological methods and identified by using molecular biological methods. Turbidimetric method was used to test the microbial biomass and the growth of strains. Results Three phenol-degradation strains, which were separated from the active sludge from a coking wastewater plant, could grow in a culture medium in which phenol was the only carbon source, and anyone of these phenol-degradation strains could totally degrade 100 ml phenol solution whose concentration was 800 mg/L in 72 hours. After the 16 s rDNA sequences of these strains were checked and these strains were compared with the known strains, a result was got that these three phenol-degradation strains belonged to Halobacillus sp., Pseudomonas sp., Rhodococcus sp. respectively. Conclusion The three efficient phenol-degradation strains got in the present study are applicable to the biological treatment of wastewater from coking plants.