硝基化合物的高效液相色谱(HPLC)分析

Studies on the HPLC analysis conditions of nitryl compounds

硝基化合物是炸药废水的主要成分,大部分硝基化合物具有较高的毒性.本文对奥克托今、黑索今、1,3,5-三硝基苯、1,3-二硝基苯、硝基苯、2,4,6-三硝基甲苯、2-氨基-4,6-二硝基甲苯、2,4-二硝基甲苯共8种硝基化合物的紫外光谱和液相色谱分离条件进行了研究,其最佳检测波长分别为228 nm、227 nm、227 nm、237 nm、272 nm、230 nm、226 nm、244 nm.本文建立了8种硝基化合物的高效液相色谱测定方法,色谱条件为:色谱柱为ZORBAX SB-C18(3.0 mm×250 mm,5μm),检测器为紫外检测器,流动相为甲醇-水(50∶50),流速为0.5 mL.min-1.水中8种硝基化合物可以在13 min内得到较好的分离,检出限均≤0.8 ng,回收率大于95%.

Nitro compounds are the main components of explosive wastewater.Most nitro compounds are poisonous.This paper studied the ultraviolet spectra and HPLC separation conditions of eight nitro compounds,including HMX,RDX,1,3,5-trinitrobenze,1,3-dinitrobenzeze,Nitrobenzene,TNT,2-Amino-4,6-dinitrotoluene and 2,4-dinitrotoluene.Their optimal determination wavelengths are 228 nm,227 nm,227 nm,237 nm,272 nm,230 nm,226 nm,244 nm respectively.The HPLC method is employed with ZORBAX SB-C18 column（3.0 mm×250 mm,5 μm） and a UV detector.The mobile phase was methanol mingled with water（Vmethanol∶Vwater=50∶50） at a flow rate of 0.5 mL·min-1.The eight nitro compounds can be separated well in 13 min,the detection limits are less than 0.8 ng and the recoveries are more than 95%.