癌性胸腹水MTT法抗癌药物敏感性试验研究

Anticancer Drug Sensitivity Test of Malignant Plcural and Ascitic Fluid with MTT Colorimetric Method

目的 采用MTT比色法测定 39例癌性胸水及 2 7例癌性腹水对 10种常用化疗药物敏感性。方法 用常规方法收集胸腹水 ,并用肝素抗凝 (10u ml) ;不连续密度梯度离心法去除渗出液中血细胞 ,制成肿瘤单细胞悬液 ,进行药敏试验。结果 观察到肺癌患者的实体瘤组织与胸水之间肿瘤细胞相对抑制率有较好的相关性。同时观察到胸腹水中大量血细胞对药敏试验结果有较大影响 ,应预先除去。结论 根据药敏结果进行胸腹腔给药治疗 ,效果较好 ,80 %患者胸腹水近期得到控制 ,对于延长患者生命有重要意义。

Chemosensitivity of 39 samples of malignant ascitic fluid to 10 anticancer drugs were assayed in vitro using MTT colorimctric method.Malignant plural fluid or ascitic fluid was collected in sterilized bottles containing 10 units of haparin for each milliliter of malignant fluid.Density gradient centrifugation was used to separate malignant cell from blood stained effusions.Single cell suspensions were prepared and tumor cell inhibition rates were determined by MTT assay.It was shown that there was no statistically significant difference in anticancer drug sensitivity between the solid tumor and malignant fluid affected anticancer drugs sensitivity and must be seperated.By intraplcural of intraascitic injection of sensitive drugs,80% of these cancer patients obtained a short term improvement.