神经肽YY_1受体样免疫反应物质在大鼠冠状血管中的分布

Distribution of neuropeptide Y Y_1 receptor like immunoreactivity in rat coronary blood vessels

目的 对大鼠冠状血管中神经肽 Y(NPY)的 Y1 受体样免疫反应物质 (- L I)进行定位 .方法 用免疫组织化学方法在光镜和电镜水平进行观察 .结果 大鼠心脏内大的冠状动脉未见 Y1 受体免疫反应的 (- Ir)标记 ,小动脉和微动脉的多数平滑肌细胞则含 Y1 受体 - L I.小动脉和微动脉平滑肌细胞的 Y1 受体 - L I在血管外膜侧、内皮细胞侧和两个平滑肌细胞相邻侧的细胞膜上均有散在的 Y1 受体 - L I和 Y1 受体 - Ir小泡 ,一些毛细血管的内皮细胞呈 Y1 受体 - L I.结论 NPY对冠状血流的调节主要作用于小动脉和微动脉的平滑肌细胞 ,有些毛细血管的内皮细胞可能也参与 NPY对血流的调节 .

AIM To localize neuropeptide Y Y 1 receptor like immunoreactivity ( R LI) in rat coronary blood vessels. METHODS Immunohistochemistry at both light and electron microscopic levels was used in the study. RESULTS Y 1 R LI was not found in large coronary arteries of the rat. However, most smooth muscle cells (SMC) in small arteries and arterioles were Y 1 R positive. Y 1 R LI and Y 1 R Ir small vesicles were on the cellular membrane of the outersurface and the endothelial side of SMC, and were often lateral on the interface between two SMC. Furthermore, there was Y 1 R LI in the endothelium of some capillary blood vessels. CONCLUSION SMC of small arteries and arterioles play an important role in the regulation of coronary blood flow by NPY, and some endothelium of the capillary blood vessels may be involved in the regulation of blood flow.