HPLC法测定姜状三七根茎中的人参皂苷Rg_1、人参皂苷Rb_1和三七皂苷R_1

Determination of notoginsenoside R_1、ginsenoside Rg_1 and ginsenoside Rb_1 in Panax zingiberensis by HPLC

建立人参属植物姜状三七根茎中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的含量测定方法.采用HPLC法,色谱柱为Phenomenex C18(250 mm×4.6 mm,5μm);流速为1.0 mL/min;检测波长203 mm;柱温30℃;流动相为乙腈-水(梯度洗脱).三七皂苷R1的线性范围为0.315～1.575μg(r=0.999 1),平均回收率为三七皂苷R1101.4%,RSD=1.79%;人参皂苷Rg1的线性范围为1.203～6.015μg(r=0.999 8),平均回收率为人参皂苷Rg198.54%,RSD=1.90%;人参皂苷Rb1的线性范围为0.276～1.38μg(r=0.999 6),平均回收率人参皂苷Rb1102.10%,RSD=1.53%.所建方法简便、准确、重复性好,可同时测定姜状三七根茎中人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的含量.

A method is established for the determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.A HPLC method was adopted.The analysis was carried out on all analytical column C18（50 mm×4.6 mm,luna 5 μm）.The flow rate was 1.0 mL·min-1 and the detective wavelength was set at 203 mm with column temperature of 30 ℃.The mobile phase consisted of acetonitrile-water（gradient elution）.The linear range was 0.315—1.575 μg（r=0.999 1）and the average recovery was 101.4%with the RSD of 1.79% for notoginsenoside R1.The linear range was 1.203—6.015 μg（r=0.999 8）and the average recovery was 98.54%with the RSD of 1.9% for ginsenoside Rg1.The linear range was 0.276—1.38 μg（r= 0.999 6）and the average recovery was 102.1%with the RSD of 1.53% for ginsenoside Rb1.The method is simple,reproducible and suitable for determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rb1 in Panax zingiberensis.