T(4-Mop)PS_4卟啉与牛血清蛋白相互作用的研究

Study on the Interaction of T(4-Mop)PS_4 with BSA

以四-(4-甲氧基-3-磺酸基苯)卟啉(T(4-Mop)PS4)为探针,通过T(4-Mop)PS4与牛血清白蛋白(BSA)的相互作用,建立了测定BSA的电化学分析方法。T(4-Mop)PS4的峰电流变化(ΔIp)与BSA在2.0×10-6～1.0×10-5mol.L-1范围内呈良好的线性关系,检出限为1.2×10-6mol.L-1;对5.0×10-6mol.L-1BSA平行测定8次,其相对标准偏差为2.0%,回收率为95%～104%。组氨酸、缬氨酸、苯丙氨酸、丝氨酸、异白氨酸、谷氨酰胺、苏氨酸等氨基酸对BSA的测定不产生干扰。采用紫外可见光度法、荧光光谱法和线性扫描伏安法(LSV)研究了T(4-Mop)PS4与BSA之间的相互作用,并测定了二者相互作用的结合常数和结合比。研究表明,T(4-Mop)PS4与BSA之间主要以疏水作用力结合,形成了1∶1的稳定复合物。

Using meso-terakis （4-methoxyl-3-sulfonatophenyl） porphyrin （ T （4-Mop） PS4 ） as an elec- trochemical probe, a new eleetroanalytical method for the determination of bovine serum albumin （BSA） was established based on the interaction of BSA with T（4-Mop）PS4. A good linear relation- ship was obtained between the variation of peak current and concentration of BSA in the range of 2. 0 × 10^-6 -1.0 × 10^-5 mol × L^-1. The limit of detection（LOD） was 1.2 × 10^-6 mol · L-j and the relative standard deviation（ RSD, n = 8） for 5.0 × 10^-6 mol · L^-1 BSA was 2.0% . The supramolecular complex of T（4-Mop）PS4 with BSA was studied by three different methods, including spectrophotom- etry, fluorometry and linear sweep voltammetric （LSV） methods. The binding ratio and binding con- stant of T（4-Mop）PS4 with BSA were obtained. The experimental data demonstrated that T（4-Mop） PS4 could form a 1 ： 1 stable supramoleeular complex with BSA, and hydrophobic interaction is the main reaction between them.