气相色谱法估算多环芳烃半数致死浓度

The estimation of median tolerance limit of polynuclear aromatic hydrocarbons by gas chromatography

用气相色谱相对比保留体积Ｖｇ（ｒ）估算多环芳烃ＰＡＨｓ在鱼体内９６ｈ的半数致死浓度（ＬＣ５０）.测定了麦穗鱼对萘、联苯、β－甲基萘、２，３一二甲基萘、２，７一二甲基萘、苊、菲７种ＰＡＨｓ的ＬＣ５０，建立了６种不同极性色谱固定相上的ＬＣ５０与Ｖｇ（ｒ）的一元回归方程和ＬＣ５０、Ｖｇ（ｒ）、一阶分子连接性指数'Ｘ的二元回归方程，统计检验表明，两类回归方程总体显著相关，巨相关性不受固定相极性的影响用Ｖｇ（ｒ）估算ＰＡＨｓ对水生生物ＬＣ５０是一种估算精度高，可操作性强的好方法．

A method of high accuracy and strong operationality for estimating median tolerrance limit (LC50) in 96 hours of polynuclear aromatic hydrocarbons (naphthalene,bipheny 1, β-methy 1 -naphtalene,2,7 -dimethy 1 yl-naphthalene, acenaphthene and phenathrene) in Pseudorasbora Parva is discussed. In this method,the gas chroniatographic parameter of Vg(r),relative specific retention volume,is used to estimate LC50, in Pseudorasbora Parva. Both the monodic regression equation of Vg(r) and LC50 and the binary regression equation of LC50'Vg(r) and molecular connectivity index ('X) on stationary phases of six kinds of different polarity are established. Statistical examination shows that the significant correlation is present for both regression equations and their correlation is not affected by the polarity of the stationary phases.