心房颤动患者循环microRNAs表达谱的初步研究

Preliminary study of circulating microRNA expression profiles in patients with auricular fibrillation

目的利用microRNA(miRNA)芯片研究持续性和阵发性心房颤动(房颤)患者循环miRNAs表达谱的改变,为进一步探讨miRNA对房颤的调控机制提供依据。方法解放军总医院心内科2010年11月—2011年2月持续性房颤患者、阵发性房颤患者和健康对照者各5例,取其全血标本,提取血清总RNA,采用microRNA芯片进行杂交,得到miRNA表达谱,通过Volcano Plot方法寻找差异表达的miRNAs,并采用MEV软件进行聚类分析。结果和健康对照者相比,阵发性和持续性房颤患者血清中表达都有明显差异的miRNA共有13个,其中表达上调的有8个:miR-3169,miR-3612,miR-634,miR-376a,miR-517b,miR-377*,miR-590-3p,miR-664;表达下调的有5个:miR-1,kshv-miR-K12-5,miR-378c,miR-204,miR-27a。阵发性房颤和持续性房颤患者间miRNA的表达谱也有显著差异。结论持续性和阵发性房颤患者循环miRNAs表达谱有显著改变,提示循环miRNAs可用于房颤发生和发展过程中调控机制的研究。

Objective To investigate the expression profiles of the circulating miRNA of patients suffering from persistent and paroxysmal auricular fibrillation（AF） by using microRNA（miRNA） chips.This study will provide the basis for further investigation of the regulatory mechanisms of miRNAs in auricular fibrillation.Methods A total of 5 patients with persistent AF,5 patients with paroxysmal AF,and 5 healthy volunteers were enrolled for study in the Cardiology Department of the Chinese People＇s Liberation Army General Hospital.Their whole blood specimens were taken to extract the total RNA serum.MicroRNA chips were used for hybridization,and then miRNA expression profiles were obtained.The volcano plot method was adopted to search for differentially expressed miRNAs,and MEV software was used to conduct clustering analyses.Results Compared with the healthy control group,13 miRNA models exhibited significant differential expressions in the serum from persistent and paroxysmal auricular fibrillation patients.Among the 13 models,eight 8 were upregulated,namely,miR-316,miR-3612,miR-634,miR-376a,miR-517b,miR-377＊,miR-590-3p,and miR-664,and five were downregulated,namely,miR-1,kshv-miR-K12-5,miR-378c,miR-204,and miR-27a.A difference in miRNA expression profiles between patients with persistent and paroxysmal AF was discovered.Conclusions The circulating miRNA expression profiles exhibited significant changes,and the study showed that circulating miRNA could be used to study the regulatory mechanisms in the occurrence and development of AF.