3例不同硒反应症状人血浆和血红细胞蛋白中硒的形态分布

Selenium distribution in proteins of three representative human plasmas and erythrocytes in China

用SDSPAGEHPLC荧光检测方法研究了3例人血浆和红细胞蛋白或其亚基中的硒分布.在所采集的硒中毒病人,无病人和大骨节病人血浆中,分别检出5,5和4条含硒条带,在红细胞中则分别检出4,4和3条含硒条带.人血浆白蛋白或血浆硒蛋白P(SeP)和血红蛋白是人血硒的优势结合蛋白.另外,3个样品的血浆中517—434kDa部分条带可能是含硒蛋白,红细胞中分子量小于143kDa的可能是含硒肽分子.对这些条带的归属及其与地区硒水平之间的关系进行了讨论.

Selenium distribution in proteins of three representative human plasmas and erythrocytes was evaluated by using SDS\|PAGE separation of proteins and HPLC\|fluorometric determination of selenium. These three samples were obtained from volunteers lining in three regions:seleniferous region (with the symton of selenium intoxication),Se\|adequate region (Beijing) and Se\|deficient region (with the sympton of Kaschin\|Beck disease).Selenium can be detected in 5,5 and 4 protein bands in human plasma and 4,4 and 3 protein bands in human erythrocyte, from these three samples respectively. The major selenium\|containing protein in human plasma is albumin or selenoprotein P (Se\|P) and in erythrocyte is hemogleobin. A protein band in molecular weight of 51.7—43.3 kDa was suspected to be a new selenium containing protein in human plasma. Beside, a peptide with molecular weight smaller than 14.3 kDa with selenium incorporation was found. Discussions were given on the identification of these Se\|containing proteins with respect to the Se levels in different regions.