摘要
目的探讨胃癌组织中食管癌相关基因4(Esophageal cancer-related gene 4,ECRG4)启动子区的甲基化情况及其临床意义。方法应用甲基化特异性PCR(Methylation specific-PCR,MSP-PCR)法,检测49份胃癌组织、30份癌旁组织和15份正常组织标本中ECRG4基因启动子区的甲基化情况,并分析其与临床病理特征之间的相关性。结果胃癌组织[69.4%(34/49)]和癌旁组织[53.3%(16/30)]ECRG4基因甲基化检出率均明显高于正常组织[6.7%(1/15)](P<0.01);Ⅲ+Ⅳ期ECRG4基因甲基化检出率[80%(24/30)]明显高于Ⅰ+Ⅱ期[52.6%(10/19)](P<0.05),表明ECRG4基因甲基化检出率与病理分期相关,而与患者年龄、性别及淋巴结转移无相关性(P>0.05)。结论 ECRG4基因启动子区异常甲基化与胃癌的发生发展密切相关,可作为胃癌早期辅助诊断的分子标志物之一。
Objective To investigate the methylation of esophageal cancer-related gene 4（ECRG4） promoter region in gastric cancer tissue and its clinical significance.Methods The mythylation of ECRG4 gene promoter regions in 49 samples of gastric cancer tissue,30 samples of normal tissue adjacent to gastric cancer and 15 samples of normal gastric tissue were determined by methylation specific-PCR（MSP-PCR）,and analyzed for relationship to clinical pathological characters.Results The methylation rates of ECRG4 gene promoter region were significantly higher in gastric cancer tissue[69.4%（34 / 49）] and normal tissue adjacent to gastric cancer [53.3%（16 / 30）] than in normal gastric tissue [6.7%（1 / 15）]（P 0.01）,and in gastric cancer tissues at stages Ⅲ ＋ Ⅳ[80%（24 / 30）] than in those at stages Ⅰ ＋ Ⅱ[52.6%（10 / 19）]（P 0.05）.It indicated that The methylation rate of ECRG4 gene promoter region was related to the pathological stage of gastric cancer,while showed no relationship to the age and sex of patients or the metastasis of gastric cancer to lymph node（P 0.05）.Conclusion Abnormal methylation of ECRG4 gene promoter region was closely related to the onset and progress of gastric cancer,which might be used as a molecular maker for early assistant diagnosis of gastric cancer.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第3期353-356,共4页
Chinese Journal of Biologicals
基金
辽宁省自然科学基金课题(201102131)
