载脂蛋白E和血红素加氧酶-1在人脑胶质母细胞瘤中的表达及其意义

Expressions and roles of apoprotein E and heme oxygenase-1 in human brain glioblastoma

目的研究载脂蛋白E(Apoprotein E,ApoE)和血红素加氧酶-1(Heme oxygenase-1,HO-1)在胶质母细胞瘤中的表达,并探讨二者在胶质母细胞瘤发生发展中的作用。方法收集人脑胶质母细胞瘤患者肿瘤组织标本41份及胶质母细胞瘤旁脑组织标本14份,采用免疫组化法检测组织标本中ApoE和HO-1蛋白的表达情况,分析ApoE和HO-1的表达与患者临床病理特征之间的相关性。结果 ApoE和HO-1蛋白在胶质母细胞瘤中表达的阳性率(82.9%和92.7%)与瘤旁正常组织(均为7.1%)相比,差异均有统计学意义(P均<0.001),且ApoE与HO-1的表达呈正相关(rs=0.321,P<0.05);患者性别、年龄、术前KPS评分、手术切除范围、肿瘤大小、术后放化疗情况与ApoE、HO-1蛋白的表达无相关性。结论 ApoE和HO-1与胶质母细胞瘤的发生发展密切相关,为判断胶质母细胞瘤的预后及治疗提供了新的参考。

Objective To investigate the expressions of apoprotein E（ApoE） and heme oxygenase-1（HO-1） in human brain glioblastoma as well as their roles in onset and progress of glioblastoma.Methods Forty-one samples of human brain glioblastoma and 14 samples of normal tissue adjacent to glioblastoma were collected and determined for expressions of ApoE and HO-1 by immunohistochemical assay,based on which the relationship of ApoE and HO-1 expressions to clinical pathological characters of patients was analyzed.Results Both the expression levels of ApoE and HO-1 were significantly in glioblastoma（82.9% and 92.7% respectively） than in normal tissue adjacent to glioblastoma（each 7.1%）（each P 0.001）.The expression of ApoE was positively related to that of HO-1（rs = 0.321,P 0.05）.However,the expressions of ApoE and HO-1 showed no relationship to the sex,age,KPS score before surgery,range of resection,tumor size and radiotherapy after surgery.Conclusion ApoE and HO-1 were closely related to the onset and progress of glioblastoma,which provided a new reference for judgment of prognosis and treatment of glioblastoma.