旋转刺激对大鼠七氟烷麻醉敏感性的影响

Effect of rotation stimulation on the anesthetic sensitivity of sevoflurane in rats

目的:探讨航海刺激对大鼠七氟烷麻醉敏感性的影响,为海上环境下七氟烷的合理使用提供依据。方法:以大鼠条件性厌食症作为判断指标,采用仿制Crampton旋转刺激器制作大鼠运动病模型。①60只SD大鼠随机分为对照组(n=15)和旋转组(n=45),观测2组大鼠的活动和行为变化、达到睡眠和麻醉状态所需七氟烷浓度以及麻醉苏醒时间。②32只SD大鼠随机分为四组(n=8):对照(Ⅰ)组、旋转(Ⅱ)组、麻醉(Ⅲ)组和旋转后再麻醉(Ⅳ)组,分别检测4组大鼠之大脑皮层、下丘脑和海马组织的乙酰胆碱、去甲肾上腺素、г-氨基丁酸及谷氨酸含量。结果:对照组大鼠达到睡眠和麻醉状态所需七氟烷浓度分别为1.74%±0.05%和3.54%±0.05%,旋转组分别为1.51%±0.06%和3.14%±0.08%,旋转组大鼠达到睡眠和麻醉状态所需七氟烷浓度明显降低(P<0.01)。神经递质变化以Ⅲ组大鼠各递质含量显著降低为其主要特点,在大脑皮层和海马组织中表现尤为明显(P<0.01)。Ⅱ组大鼠大脑皮层Ach显著升高,海马组织NE、GABA明显降低。Ⅳ组变化趋势更接近于Ⅱ组,即以旋转刺激的影响更为明显。结论:模拟航海刺激可明显增加大鼠对七氟烷麻醉的敏感性。

Objective： To explore the effect of simulated navigation stimulation on the anesthetic sensitivity of sevoflurane in rats,so as to provide basis for rational using sevoflurane during navigation.Methods： SD rats were stimulated by Crampton model and the conditioned taste aversion（CTA） was regarded as criterion of motion sickness.① 60 rats were randomly divided into control（n=15） and rotation group（n=45）.The changes of behavior and autonomic activity,sevoflurane concentration achieved sleep and anesthesia states,and the revitalization time were observed in two group rats.② 32 rats were randomly divided into control（I）,rotation（II）,anesthesia（III） and rotation plus anesthesia（IV） group（n=8）.The acetylcholine（Ach）,norepinephrine（NE）,г-aminobutyric acid（GABA）,glutamic acid（Glu） of brain cortex,thalamus and hippocampus were determined respectively in the four group rats.Results： In control group,the sevoflurane concentration achieved sleep and anesthesia states were 1.74%±0.05% and 3.54%±0.05% respectively,but,those concentrations were 1.51%±0.06% and 3.14%±0.08% in rotation group.There were lower significantly in rotation group than those in control group（P0.01）.It was a major characteristic that all of the neurotransmitters were reduced significantly in Ⅲ group,this was even more in brain cortex and thalamus（P0.01）.In Ⅱ group,Ach was upward in brain cortex,NE and GABA were reduced in hippocampus obviously.The change tendency of neurotransmitters in Ⅳ group was more close to Ⅱ group,that was,the effect of rotation stimulation was more obvious.Conclusion： The anesthetic sensitivity of sevoflurane could be obvious increased in rats simulated navigation stimulation.