豚鼠耳蜗螺旋动脉平滑肌细胞和内皮细胞静息膜电位特性

The characteristics of resting membrane potential on smooth muscle cells and endothelial cells in guinea pigs cochlea spiral artery

目的：多种内耳疾病和内耳微循环障碍有关,但目前对提供内耳主要血供的耳蜗螺旋动脉平滑肌（SMC）和内皮细胞（EC）的生理学特性还不十分清楚,需要进一步研究。方法：本研究采用双细胞内微电极记录技术和细胞荧光染色技术,研究耳蜗螺旋动脉平滑肌和内皮细胞的膜电位特性和细胞间的通讯联系。结果：研究发现耳蜗螺旋动脉SMC和EC具有高、低两种静息膜电位（RP）状态,两种静息膜电位状态的细胞对乙酰胆碱和高K＋的反应完全不同。双微电极可同时记录到EC-ECS、MC-SMC和SMC-EC不同类型的细胞,两个细胞的静息膜电位也可以是双高RP、双低RP和一高一低RP。实验所记录的一高一低RP均是SMC-EC类型,而且EC初始膜电位均为高电位,SMC初始膜电位均为低电位。而双高RP和双低RP可以是SMC-SMC或EC-EC或SMC-EC类型。结论：结果表明耳蜗螺旋动脉的SMC和EC在0.3~0.5 mm的范围内,同类细胞之间有很好的通讯联系,能很好的保持功能的协同和一致,血管壁异类细胞则不同。

Objective： A variety of inner ear disease is related to microcirculation disturbance of inner ear,but smooth muscle cells（SMC） and endothelial cells（EC） of the spiral modiolar artery（SMA）,which is the main blood supply to the inner ear,physiological feature is not very clear.Methods： In this study,two-intracellular microelectrode recording technique and cell staining techniques to study the SMC and EC resting membrane potential characteristics and communication links between cells of SMA.Results： Study found that SMC and EC have high and low resting membrane potential state,two state of the resting membrane potential of cells to ACh and high K＋ response is completely different.The different types of cells,EC-EC,SMC-SMC and SMC-EC,can simultaneously record by two-microelectrode,two cell resting membrane potential can also be a double-high RP,double-low RP and one high-and one low-RP.Experiment recorded in one high and one low-RP are the SMC-EC types,and ECs initial membrane potential are high potential,SMCs membrane potential are low initial potential.The double-high and double-low RP can be SMC-SMC or EC-EC or SMC-EC types.Conclusion： The results show that SMC and EC in the 0.3～0.5 mm range,similar type of cells have very good communication,can function together to maintain good and consistent,heterogeneous cell performance is more different.